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RNA sequencing of ex vivo microtumor models embedded in different matrices and constructed from either control or Arpc4-knockout cells.

ABSTRACT: The loss of Arpc4 resulted in reduced expression of other Arp2/3 complex components, rendering the Arp2/3 complex nonfunctional. The ex vivo microtumor(3D) models were composed of either control or Arpc4 knockout (KO) KC 8025 cells embedded in different matrices containing three condition:25% collagen I, 25% collagen I + 75% Matrigel, and 75% Matrigel for 5 days. To identify genes affected by Arpc4 knockout in this model, RNA-seq analysis was performed on bulk samples. 8025 cell line was derived from primary cells isolated from genetically engineered p48Cre/+; LSL-KrasG12D/+ (KC).

ORGANISM(S): Mus musculus

PROVIDER: GSE305670 | GEO | 2025/12/21

REPOSITORIES: GEO

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