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Identification RBPMS2 as a key regulator in mouse early oocyte development

ABSTRACT: In the process of follicle formation, oocyte stably maintain large number of RNAs for development. However, how mammalian oocytes regulate these mRNAs, especially in the early oocyte, is unclear. We focused on RNA-binding proteins and identified RBPMS2 as a key factor in this process. Genetic disruption Rbpms2 causes female mouse infertility due to primordial oocyte defect. Transcriptome and RIP-seq analysis revealed that RBPMS2 binds 3′ UTR region to stabilize mRNAs of known oogenesis regulators, such as Lhx8. RBPMS2 also targets transcripts regulating cytoskeleton and cellular trafficking, such as Clasp1 and Rab27a, which are down-regulated in knock out oocytes. Rbpms2 knock out induces impairments in endocytosis and the accumulation of mitochondria and Golgi bodies in primordial oocyte. In summary, our study reveals RBPMS2 regulates mouse oogenesis through stabilizing mRNAs regulating oocyte development cellular trafficking and organelle enrichment, connecting post-transcriptional regulation to organelles accumulation and early oocyte development.

ORGANISM(S): Mus musculus

PROVIDER: GSE306544 | GEO | 2026/01/16

REPOSITORIES: GEO

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