Transcriptomics

Dataset Information

0

Organoid Modeling of Mouse Anterior Tongue Epithelium Reveals Regional and Cellular Identities


ABSTRACT: The tongue is essential for swallowing, taste perception, and mechanosensation. The anterior and posterior parts of the tongue have region-specific developmental origins and are maintained by adult epithelial stem/progenitor cells. In vitro models that can be used to investigate anterior tongue biology have been lacking. Here, we develop a protocol to generate a long-term expanding organoid model from adult mouse dorsal anterior tongue. Anterior tongue organoids consist of Lgr6+ cells, Sox2+ stem/progenitor cells, and Hoxc13+ filiform papillae progenitor cells. Furthermore, anterior tongue organoids share region-specific transcriptomic profiles, gene regulatory networks, and signaling pathways with anterior tongue tissue. Anterior tongue organoids can be differentiated into various epithelial cell types including Merkel-like cells, keratinocytes, and taste bud cells. Gene regulatory network analysis reveals transcriptional programs associated with Krt8+ cell and Krt23+/Sbsn+ keratinocyte differentiation in the organoids. Together, this study provides an in vitro model of mouse dorsal tongue epithelium.

ORGANISM(S): Mus musculus

PROVIDER: GSE307623 | GEO | 2025/09/22

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-09-10 | PXD025190 | Pride
2021-12-25 | GSE191169 | GEO
2023-02-07 | GSE207624 | GEO
2016-09-06 | E-GEOD-75648 | biostudies-arrayexpress
2016-09-06 | GSE75648 | GEO
2025-09-01 | GSE274015 | GEO
2025-09-01 | GSE274014 | GEO
2009-07-01 | GSE16485 | GEO
2010-03-25 | E-GEOD-20269 | biostudies-arrayexpress
2024-12-04 | GSE263433 | GEO