Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA-seq of MHCII-KO and WT B cells during influenza infection

ABSTRACT: To evaluate the programming of B cells subsets that arise in the absence of CD4 T cell interactions we adoptively transferred wild-type (WT) and majorhistocompatibility class II (MHC-II) knockout B cells into WT hosts and infected the mice with influenza PR8. All activated responding transferred B cells were profiled at day 14, a time point when T cell independent memory responses arise, and scRNA-seq and BCR-seq performed to understand the transcriptional programming that is dependent on CD4 T cell interactions. We identified minimal differences between WT and KO B cells, with a diverse number of memory B cell clusters identified that were populated by cells fron both cohorts of mice. These data indicate that the memory B cells that form early, likely extrafollicularly, do not depend on CD4 T cells for their programming and diversity.

ORGANISM(S): Mus musculus

PROVIDER: GSE307658 | GEO | 2026/02/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional profiling of memory B cells that form during influenza infection of majorhistocompatibility class II (MHC-II) knockout mice.

Project description:To evaluate the programming of activated B cells and memory B cell subsets that form in the absence of CD4 T cells we infected wilt-type (WT) and majorhistocompatibility class II (MHC-II) knockout mice with influenza PR8 and profiled the response at day 14, a time point when T cell independent memory responses arise. We isolated activated, dividing B cells which give rise to memory subsets as well as IgM and class-switched memory B cells from both cohorts of animals. These studies identify the memory B cell programming that is dependent on CD4 T cells.

2026-02-21 | GSE307545 | GEO

Expression data from WT and IL-2 secondary CD4 T cell effectors responding towards infuenza A virus infection

2015-05-14 | GSE60186 | GEO

Expression data from WT and IL-2 secondary CD4 T cell effectors responding towards infuenza A virus infection

Project description:How IL-2 produced by secondary CD4 T cell effectors, derived from resting memory cells, impacts memory CD4 T cell function and survival to memory following antigen re-encounter is unknown. We used microarrays to detail the global programme of gene expression underlying differences between WT and IL-2 deficient secondary CD4 T cell effectors purified from the lung on day 7 following A/PR8/34-OVAII influenza infection. Congenic primed memory DO11.10 TCR trangenic CD4 T cells were sort purifed from the lungs of infected mice on day 7 post infection for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain only CD4 T cell populations that had entered into the immune response by also sorting and collecting only those cells that had divided at least 5 times by CFSE analysis.

2015-05-14 | E-GEOD-60186 | biostudies-arrayexpress

Expression data from primary and secondary CD4 T cell effectors responding towards influenza A virus infection

Project description:How secondary CD4 T cell effectors, derived from resting memory cells, differ from primary cells, derived from naïve precursors, and how such differences impact recall responses to pathogens is unknown. We used microarrays to detail the global programme of gene expression underlying differences between primary and secondary CD4 T cell effectors purified from the spleen, dLN, and lung on day 7 following A/PR8/34 influenza infection. Congenic naïve or in vivo influenza primed memory HNT TCR trangenic CD4 T cells were sort purifed from the spleens, dLNs, and lungs of infected mice on day 7 post infection for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain only CD4 T cell populations that had entered into the immune response by also sorting and collecting only those cells that had divided at least 5 times by CFSE analysis.

2012-08-27 | E-GEOD-40230 | biostudies-arrayexpress

Expression data from primary and secondary CD4 T cell effectors responding towards influenza A virus infection

2012-08-27 | GSE40230 | GEO

Expression data from self-deprived regulatory CD4 T cells

Project description:Disruption of TCR /MHC class II interactions leads rapidly to alterations of the common CD4 Treg transcriptional signature Self-deprived, non-functional Tregs were compare to fully functional Tregs by microarrays. Total T cells from the periphery of WT mice were adoptively transferred into CD3ε-/- recipient mice lacking or not MHC class II molecule expression (MHC II- or MHC II+ recipient mice, respectively). Five days later, peripheral Tregs transferred in MHC II - competent (CD4CD25B6) or - deficient (CD4CD25IIko) recipient were purified for RNA extraction and hybridization on Affymetrix microarrays.

2011-01-20 | E-GEOD-26714 | biostudies-arrayexpress

Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts. [CAF]

Project description:A key unknown of the functional space in tumor immunity is whether physiologically relevant cancer antigen presentation occurs solely in draining lymph nodes versus tumors. Professional antigen presenting cells, i.e. the dendritic cells, are scarce and immature within tumors, greatly outnumbered by MHCII expressing non-hematopoietic cells, such as antigen-presenting cancer-associated fibroblasts (apCAFs). We hypothesized that after their exit from tumor-draining lymph nodes T cells depend on a second wave of antigen presentation provided in situ by structural cells. We show that dense apCAF regions in human lung tumors define hot immunological spots with increased numbers of CD4 T cells. The transcriptomic profile of human lung apCAFs aligned to that of pancreatic apCAFs across mice and humans and were both enriched for alveolar type II genes, suggesting an epithelial origin. Mechanistically, human apCAFs directly activated the TCRs of adjacent effector CD4 T cells and at the same time produced high levels of c1q, which acted on surface c1qbp on T cells to rescue them from apoptosis. Fibroblast-specific deletion of MHCII in mice impaired local MHCII immunity and accelerated tumor growth, while inducing c1qbp overexpression in adoptively transferred T cells expanded their numbers within tumors and reduced tumour burden. Collectively, our work shows that tumor T cell immunity post lymph node exit requires peripheral antigen presentation by a subset of CAFs and proposes a new conceptual framework upon which effective cancer immunotherapies can be built.

2021-12-13 | GSE164653 | GEO

Ongoing classical IL-6 signaling is required to maintain a pathogenic Th17 response in vivo

Project description:Th17 precursors from WT and IL6ra-/- mice were co-transferred into Rag1-/- mice to induce colitis. RNA was isolated from CD4+ T cells pre-transfer and from colonic CD4+ T cells 4 weeks post- transfer Another aim is to compare gene expression of WT (IL-6) v WT (HDS) v IL6RaKO (HDS) in vitro polarized Thy1.1+ Th17p cells

2020-07-17 | GSE115141 | GEO

Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells

Project description:The PI3K/Akt signaling pathway impacts various aspects of CD8 T cell homeostasis, such as effect versus memory cell differentiation, during viral infection. We used microarrays to determine which downstream molecules were affected and what other signaling pathways were interconnected with the Akt pathway by constitutive activation of Akt in LCMV-infected CD8 T cells. Splenocytes from naive P14/WT or P14/Akt mice were stained with anti-CD8 and anti-Ly5.1, and CD8 T cells were sorted using a FACSAria II instrument. Purified Ly5.1+ CD8 T cells from P14/WT or P14/Akt mice were transferred into B6 mice, which were subsequently infected with LCMV Armstrong. At day 8 post infection, splenocytes were stained with anti-CD8, anti-Ly5.1, anti-KLRG1, and anti-CD127. Following staining, short-lived effector cells (SLECs) and memory precursor effector cells (MPECs) were sorted using the FACSAria II instrument; the purity of the sorted cells was >95%. A total of 5 samples were analyzed, including WT naive, WT SLEC, WT MPEC, Akt naive and Akt SLEC.

2012-03-01 | E-GEOD-36168 | biostudies-arrayexpress

Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts. [CD4 T cells]

2021-12-13 | GSE164659 | GEO