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Activation of donor-derived eosinophils contributes to primary graft dysfunction during static lung graft storage

ABSTRACT: To explore the impact of antioxidant supplementation on eosinophil transcriptional responses during lung preservation, we performed bulk RNA sequencing on purified eosinophils cultured in either standard media (RPMI) or Perfadex® Plus, with or without glutathione (GSH). Cells were incubated at 37°C to mimic rewarming conditions that typically induce activation. Exposure to Perfadex® Plus resulted in reduced cell viability and suppression of eosinophil-associated transcriptional programs related to recruitment, activation, and survival. In contrast, supplementation with GSH preserved cell viability and significantly enhanced the expression of antioxidant and redox-related pathways. Enrichment analysis revealed activation of oxidative stress response, detoxification, glutathione metabolism, selenium-associated processes, and mitochondrial respiration. These findings suggest that GSH mitigates metabolic stress and dampens pro-inflammatory transcriptional activity in eosinophils, supporting its potential role in improving lung preservation outcomes.

ORGANISM(S): Mus musculus

PROVIDER: GSE308432 | GEO | 2026/09/17

REPOSITORIES: GEO

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Project description:Objective: To study the physiological role of eosinophils in the GI tract and lung under homeostatic conditions, Method: we analyzed tissue-specific eosinophil gene expression patterns by genome-wide expression microarray analysis using RNA isolated from FACS-sorted eosinophils from the small intestine or lung of naive BALB/cmice (Live eosinophils were identified as DAPI-CCR3+Siglec-F+CD45+CD4-CD8a-CD19-B220-SSChigh cells). Result: There are 513 genes that are differentially expressed by intestinal eosinophils and Lung eosinophils. Conclusion: Eosinophils from different tissues have unique gene expression patterns for distinct functions. we analyzed tissue-specific eosinophil gene expression patterns by genome-wide expression microarray analysis using RNA isolated from FACS-sorted eosinophils from the small intestine or lung of naive BALB/cmice (Live eosinophils were identified as DAPI-CCR3+Siglec-F+CD45+CD4-CD8a-CD19-B220-SSChigh cells).

2011-11-23 | E-GEOD-33807 | biostudies-arrayexpress

Microarray analysis on RNA from lung eosinophil subpopulation from naive or HDM-treated mice using Affymetrix Mouse Gene 2.0 ST

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2016-06-07 | E-MTAB-4084 | biostudies-arrayexpress

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