Dataset Information

Parasympathetic nerves in the kidney pelvis contribute to blood pressure regulation

ABSTRACT: The existence of parasympathetic innervation in the kidney has long been controversial. Recent anatomical studies have provided evidence supporting such innervation by demonstrating the presence of acetylcholine (ACh) metabolic enzymes and tracing a brain-kidney vagal axis. Although these findings confirm the anatomical pathway, the functional capacity of these nerves to release neurotransmitters remains unverified, and the physiological role of this pathway is still unknown. We delineated the innervation of the renal pelvis by co-labeling sympathetic, parasympathetic, and sensory nerves. ACh receptors expression was assessed through single-cell RNA sequencin, quantitative reverse transcription polymerase chain reaction, and fluorescent in situ hybridization. The ACh release from the renal nerves was monitored via fiber photometry with a genetically encoded ACh sensor. Optogenetic activation was employed to investigate the functional effects of renal parasympathetic efferent and afferent pathways on blood pressure and heart rate. We identified multi-component innervation in the renal pelvis, including sympathetic, parasympathetic, and sensory nerves. Moreover, muscarinic ACh receptors were found to be expressed in this region. Neurogenic ACh release from renal nerves was also detected following transmural stimulation. Furthermore, optogenetic activation of the vagal brain–kidney afferent pathway resulted in a decrease in blood pressure. These results suggest that the renal pelvis receives dual autonomic innervation from both sympathetic and parasympathetic nerves, and the vagal renal afferents are involved in blood pressure regulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE310417 | GEO | 2026/02/21

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:The gut-brain axis, a reciprocal interaction between the central nervous system (CNS) and peripheral intestinal functions, is conceptually feasible from recent clinical and experimental evidence showing mutual interactions between the CNS and gut microbiota that are closely associated with the bidirectional effects of inflammatory bowel diseases (IBDs) and CNS disorders. Despite recent advances in our understanding of neuroimmune interactions, it remains unclear how the gut and brain communicate to maintain gut immune homeostasis, including induction and maintenance of peripheral regulatory T cells (pTregs) and what environmental cues prompt the host to protect host from development of IBDs. Here, we report a novel liver-brain-gut neural arc that ensures proper differentiation and maintenance of peripheral regulatory T cells (pTregs) in the gut. The hepatic vagal sensory afferents were responsible for indirectly sensing the gut microenvironment and relaying the sensory inputs to the nucleus tractus solitarius (NTS) of the brainstem, and ultimately to the vagal parasympathetic nerves and enteric neurons. Surgical and chemical perturbation of the vagal sensory afferents at the hepatic afferent level significantly impaired colonic pTregs, which was attributed to impairment of aldehyde dehydrogenase (ALDH) expression and retinoic acid (RA) synthesis by intestinal antigen-presenting cells (APCs). Muscarinic Ach receptor (mAChR) activation directly induced ALDH gene expression both in human and mouse colonic APCs, whereas genetic ablation of mAChRs abolished APC excitement in vitro. Disruption of left vagal sensory afferents from the liver to the brainstem in colitis models reduced the colonic pTreg pool, resulting in increased susceptibility to colitis. These results demonstrate that the novel vago-vagal liver-brain-gut reflex arc tunes the number of pTregs and maintains the gut homeostasis. Intervening in this autonomic feedback feed-forward system could help develop new therapeutic strategies to treat or prevent immunological disorders of the gut.

Dataset Information

Parasympathetic nerves in the kidney pelvis contribute to blood pressure regulation

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets