Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Clip2 as a novel regulator of osteocyte maturation and morphology

ABSTRACT: The genes driving osteocyte maturation and function are not fully understood. We identified CD61 (integrin beta-3) as a marker of osteocyte maturation and developed a flow cytometry-based assay to quantify surface CD61 protein levels. Surface CD61 expression increases during osteocyte maturation, and CD61-high cells express higher levels of osteocyte marker genes. In a genome-wide screen, we identified Clip2, which encodes a microtubule binding protein, as one of dozens of genes necessary for CD61 expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE311295 | GEO | 2026/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Clip2 as a novel regulator of osteocyte maturation and morphology

Project description:Clip2 as a novel regulator of osteocyte maturation and morphology

| PRJNA1368893 | ENA

Role of Piezo1-mediated mechanotransduction in osteocyte differentiation

Project description:Mechanical force is a fundamental regulator of bone development and homeostasis. Mechanosensitive osteocytes are the most abundant bone cells that form interconnected dendrites to respond to mechanical stimuli and interact with the bone-forming osteoblasts and the bone-remodeling osteoclasts. However, the molecular mechanisms underlying osteocyte maturation and dendrite formation remain unclear. By generating a Piezo1 "knock-out" osteocyte cell line, we identified a key role of Piezo1-mediated mechanotransduction in osteocyte differentiation. QRT-PCR analysis revealed delayed osteocyte differentiation in the Piezo1 KO cells relative to WT cells. By performing bulk RNA sequencing of WT and Piezo1 KO OCY454 cells at early (D1), intermediate (D14), and late (D28) stages of differentiation allowed for the identification of key signaling pathways in driving normal osteocyte dfiferentiation, as well as those regulated by Piezo1 mechanotransduction.

2024-09-20 | GSE277264 | GEO

The osteocyte transcriptome during post-natal skeletal maturation in male and female mice

Project description:These data examine the total-RNA transcriptome of primary osteocytes in both genders at 4 different stages of skeletal maturation. These stages are prepuberty (4 weeks), post puberty (10 weeks), early skeletal maturation (16 weeks), established skeletal maturation (26 weeks). These data were used to identify temporal changes in gene expression between sexes in the osteocyte network during post natal skeletal growth and maturation.

2020-12-16 | E-MTAB-7447 | biostudies-arrayexpress

Proteomic Analysis of Plasma Membrane Proteins in Atp6ap2 (PRR)-Deficient Osteoblast-Lineage Cells

Project description:ATP6AP2 (also known as PRR) is required for osteocyte maturation and cortical bone development. To identify downstream effectors of ATP6AP2, plasma membrane-enriched proteins were isolated from control and Atp6ap2-deficient osteoblast-lineage cells and analyzed by mass spectrometry. Comparative proteomic analysis identified MMP14 as a major ATP6AP2-regulated membrane protein. These data support a role for ATP6AP2 in regulating MMP14 surface localization and osteocyte development.

2026-06-22 | PXD079754 | Pride

TF ChIP-seq from osteocyte (ENCSR300DWM)

Project description:CTCF ChIP-seq on osteocyte originated from osteocyte For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-05-23 | GSE175159 | GEO

Control ChIP-seq from osteocyte (ENCSR702WFF)

Project description:Control ChIP-seq on osteocyte originated from osteocyte For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-03-29 | GSE169778 | GEO

Osteoblast and Osteocyte Profiles at Different Cell-cummunication Status

Project description:Type of Experiment: 1) Profiles of Osteoblast vs. osteocyte in vitro; 2) Profiles of osteoblast low density vs. confluency in vitro; 3) Profiles of osteocyte with gap junction vs. without gap junction. Experimental factors: 1) 2T3 osteoblast cells at low density expressed extensive filopodia, reminiscent of early osteoblast precursors and similar to MLO-Y4 dendritic processes. 2) MLO-Y4 osteocytes at low vs. high density represent the genes that are changed in a highly connected network vs. low connected network. The number of hybridizations performed: Triplicate hybridizations for each status. Keywords = Osteoblast Keywords = Osteocyte

2010-06-09 | E-GEOD-2234 | biostudies-arrayexpress

High-Resolution Profiling of Osteocyte Transcriptomes via Single-Nucleus RNA Sequencing

Project description:We developed a protocol for snRNA-seq on bone tissue, achieving high-yield recovery of osteocyte nuclei from snap-frozen, marrow-flushed long bones. This approach minimized dissociation bias and enhanced osteocyte representation. We applied this robust method to long bones from young adult male and female mice, generating a high-resolution map of osteocyte gene expression under physiological conditions. Compared to scRNA-seq datasets, where osteocytes represent only 0.18–6.64% of cells, our snRNA-seq approach increased osteocyte capture and transcriptomic fidelity to 18.5%. We identified an osteocyte transcriptomic signature highlighting the top 30 genes, including Sost, which is typically undetected or lowly-expressed in scRNA-seq. Notably, 23 of these genes have not been well-characterized in osteocytes, including Tg, Kcnq5, Rapgef4os1, Cacna1a, Egr3, Dok5, and Lgr6, which may represent novel regulators of osteocyte biology. This study represents the first application of snRNA-seq specifically for osteocyte analysis in bone tissue, providing a valuable resource for investigating osteocyte biology and skeletal disorders.

2026-03-25 | GSE325591 | GEO

Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis.

Project description:Osteocytes are the main cells in mineralized bone tissue. Elevated osteocyte apoptosis has been observed in lytic bone lesions of patients with multiple myeloma. However, their precise contribution to bone metastasis remains unclear.Here, we investigated the pathogenic mechanisms driving melanoma-induced osteocyte death. Both in vivo models and in vitro assays were combined with untargeted RNA sequencing approaches to explore the pathways governing melanoma-induced osteocyte death. We could show that ferroptosis is the primary mechanism behind osteocyte death in the context of melanoma bone metastasis. HMOX1 was identified as a crucial regulatory factor in this process, directly involved in inducing ferroptosis and affecting osteocyte viability. We uncover a non-canonical pathway that involves excessive autophagy-mediated ferritin degradation, highlighting the complex relationship between autophagy and ferroptosis in melanoma-induced osteocyte death. In addition, HIF1α pathway was shown as an upstream regulator, providing a potential target for modulating HMOX1 expression and influencing autophagy-dependent ferroptosis. In conclusion, our study provides insight into the pathogenic mechanisms of osteocyte death induced by melanoma bone metastasis, with a specific focus on ferroptosis and its regulation. This would enhance our comprehension of melanoma-induced osteocyte death.

2024-11-24 | GSE276370 | GEO

Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis II

2024-11-24 | GSE276373 | GEO