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Single-Cell Transcriptomics Reveals the Characteristics of Bone Marrow Cellular Heterogeneity After Intramedullary Nail Implantation in Osteoporotic Fractures

ABSTRACT: After fracture, the femoral bone marrow microenvironment in mice initiates a series of reparative responses. The spatiotemporal coordinated regulation of gene expression not only determines the activation characteristics of hematopoietic and mesenchymal progenitors but also directly regulates the dynamic remodeling of various functional cell populations in the bone marrow microenvironment and the execution of bone repair functions. Historically, studies on the effects of intramedullary implant materials on the bone marrow microenvironment post-fracture have mostly relied on histological analysis of bulk tissues or functional assays of pre-selected isolated cell subsets. These approaches fail to accurately capture the heterogeneous transcriptional programs of individual cells, especially the dynamic transcriptional changes of cells in the bone marrow and on the implant surface after intramedullary nail placement. To systematically characterize the changes in the femoral bone marrow microenvironment after fracture and clarify the heterogeneity and dynamic transcriptional changes of cells in the bone marrow and on the implant surface following intramedullary nail placement, we employed single-cell mRNA sequencing (scRNA-seq) to profile the transcriptional landscapes of femoral bone marrow samples and implant-adherent cells from fracture mice implanted with intramedullary nails of different materials. This technique provides core data support for elucidating the molecular mechanisms underlying material-regulated cell differentiation trajectories and functional remodeling during fracture healing.

ORGANISM(S): Mus musculus

PROVIDER: GSE313141 | GEO | 2026/03/26

REPOSITORIES: GEO

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