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Enhanced woody biomass production in hybrid aspen through xylem-specific overexpression of Arabidopsis thaliana LBD genes

ABSTRACT: Woody biomass is a critical renewable resource for sustainable energy and material production, yet strategies to increase its yield while simultaneously improving quality remain limited. Here, we demonstrate that xylem-specific overexpression of Arabidopsis thaliana LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors markedly enhances stem secondary growth and alters wood composition in hybrid aspen (Populus tremula × Populus tremuloides Michx., clone T89). Using the LMX5 promoter to drive targeted expression of A. thaliana LBD4 and LBD11 in the developing xylem, we observed substantial stimulation of secondary xylem formation. Histological analyses revealed distinct roles for each gene: LBD4 primarily promoted xylem development through increased cell expansion, whereas LBD11 enhanced both cell proliferation and expansion. This dataset was used to analyze the LBD overexpression lines

ORGANISM(S): Populus tremula x Populus tremuloides

PROVIDER: GSE313477 | GEO | 2026/04/01

REPOSITORIES: GEO

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Similar Datasets

Populus metacaspases - The function of two type II metacaspases in woody tissues of Populus trees

Project description:Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in the xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs of hybrid aspen (Populus tremula x tremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNAi construct for PttMC13 and PttMC14. The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins as well as novel targets, such as homologs of the PUTATIVE ASPARTIC PROTEASE 3 (PASPA3) (PASPA) and the cysteine protease RD21 by PttMC13 and PttMC14. We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in controlling not only xylem cell autolysis but also cell death. This work provides a valuable reference dataset on xylem specific metacaspase functions for future functional and biochemical analyses.

2018-01-02 | PXD007958 | Pride

Proteomic analysis in transgenic hybrid aspen (Populus tremula x tremuloides) trees overexpression of vesicle-associated membrane protein PttVAP27-17

Project description:In this study we report on transgenic hybrid aspen (Populus tremula x P. tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after five years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula x tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 (Potri.019G116400) that was selected from a gene-mining program for novel regulators of wood formation. A proteomic analysis was performed to characterize the overall effect of the overexpression of PttVAP27-17 on plant metabolic pathways. 20 mg of xylem sample for the selected wild type (WT) and three transgenic lines (lines 1,2 and 3) was collected in the following manner from two-months-old, greenhouse grown trees: The frozen bottom-part of the stem (10-17 cm portion from the base of the stem) was peeled, and the surface of the secondary xylem consisting of living vessels and fibers (into the depth of approximately one mm from the surface) was scraped. The xylem scrapings were ground to fine powder in liquid nitrogen and stored at -80oC. The analyses included 3-5 biological replicates for each of the transgenic lines, and seven replicates for the wild type.

2021-09-10 | PXD023826 | Pride

Hybrid aspen (Populus tremula x tremuloides) cambial zone RNA-sequencing with 35um wide fraction scheme

Project description:Aim of the project: Genome wide gene expression profiles across the cambial zone are analyzed in 35um resolution from wild type hybrid aspen (Populus tremula x tremuloides) and two independent LMX5::AtIPT7 over expressor transgenic Populus tree lines.

2016-07-14 | E-MTAB-4631 | biostudies-arrayexpress

Thermospermine-induced transcriptomic changes in Populus stem

Project description:Thermospermine-induced transcriptomic changes were explored in Populus tremula x P. tremuloides. Transgenic hybrid aspen plants expressing 35S::POPACAULIS5 were compared to wild-type hybrid aspen under the influence of PGRs and depleted from PGRs.

2015-06-01 | GSE45879 | GEO

24-hour herbivory exposure of VHb-expressing Populus tremula x Populus tremuloides

Project description:The expression of stress-related genes induced by feeding of chestnut moth larvae (Conistra vaccinii L.) was studied with Vitreoscilla hemoglobin-expressing (VHb) and non-transgenic hybrid aspen lines (Populus tremula x P. tremuloides). Besides the herbivore-injured leaves (L1), cDNA microarray analyses were conducted using uninjured leaves of hybrid aspen lines positioned above (A) and below (B) the herbivory exposed leaves.

2013-04-24 | GSE46331 | GEO

Hybrid aspen (Populus tremula x tremuloides)1h cytokinin induction and 1h control treatment data.

Project description:Aim of the project: Genome wide gene expression analysis for cytokinin (100nM 2ip, 20nM NaPi buffer) fast (1h) response genes from 3 months old Hybrid aspen(Populus tremula x tremuloides)apical part (30 cm from tip; diameter:4-5mm) of stem. Stems of two individuals (trees 15 and 16) was cut into 50-100um thick (free hand)cross sections randomly selected stem discs are set to two pools: cytokinin treatment and control treatment. Samples are collected noon time (11:00-11:30). Cytokinin treatment: stem discs (several hundred) are submerged with 100nM 2ip, 20nM NaPi buffer, with modest shaking for 60 min +/- 2 min (time to collect about 30mg stem discs (several dozens) for RNA sample. Control treatment: identical to cytokinin treatment, only without 100nM 2ip.

2016-07-14 | E-MTAB-4635 | biostudies-arrayexpress

A multi-omics approach reveals functions of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees

Project description:Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved proteins that are involved in membrane trafficking. An integrated approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. The metabolomics analyses were performed in woody tissues of transgenic Populus trees carrying an RNAi construct for Populus tremula x tremuloides SCAMP3 (PttSCAMP3; Potri.019G104000). The secondary xylem of the transgenic trees displayed increases in the amounts of polysaccharide derived sugars as well as lignin oligomers, indicating increased deposition of both carbohydrate and lignin polymers in the secondary cell walls. Somewhat surprisingly, the transgenic lines showed significantly increased amounts of suberic acid in the secondary xylem tissues. Populus SCAMP proteins were shown to influence accumulation of secondary cell wall components including polysaccharides, polyphenolics, lipids and suberin in the woody tissues of Populus tree stems.

2018-01-08 | MTBLS478 | MetaboLights

Long term effect of nitrate treatment in hybrid aspen wood

Project description:Long term nitrogen effect in hybrid aspen (Populus tremula x P. tremuloides)

| PRJEB62128 | ENA

Comparison of 2 aspen genotypes having similar carbon assimilation rates but different allocation patterns under elevated CO2

Project description:• Reference to published study making use of this data: • Cseke LJ, Tsai C-J, Rogers A, Nelsen MP, White HL, Karnosky DF, Podila GK. (2009) Transcriptomic comparison in the leaves of two aspen genotypes having similar carbon assimilation rates but different allocation patterns under elevated CO2. New Phytologist. submitted. • This study compared the leaf transcription profiles, physiological characteristics, and primary metabolites of two Populus tremuloides genotypes (clones 216 and 271) known to differ in their responses to long-term elevated [CO2] (e[CO2]) at the Aspen FACE site near Rhinelander, WI. • Physiological responses of these clones are similar in photosynthesis, stomatal conductance, and leaf area index under e[CO2] yet very different in growth enhancement (0-10% in clone 216; 40-50% in clone 271). While few genes responded to long-term exposure to e[CO2], the transcriptional activity of leaf e[CO2]-responsive genes was distinctly different between the clones, differentially impacting multiple pathways during both early and late growing seasons. • Analysis of transcript abundance and carbon/nitrogen biochemistry suggests that the CO2-responsive clone (271) partitions C into pathways associated with active defense/response to stress, carbohydrate/starch biosynthesis and subsequent growth. The CO2-unresponsive clone (216) partitions C into pathways associated with passive defense (e.g. lignin, phenylpropanoid) and cell wall thickening. • This study indicates that there is significant variation in expression patterns between different tree genotypes in response to long-term exposure to e[CO2]. Consequently, future efforts to improve productivity or other advantageous traits for carbon sequestration should include an examination of genetic variability in CO2 responsiveness. Keywords: Tree genotype comparison under elevated [CO2]

2009-03-31 | GSE14881 | GEO

Transcriptome profiles and comparison of Arabidopsis thaliana woody (soc1-ful) and WT (Col-0) stems.

Project description:The douple mutant Arabidopsis thaliana soc1 ful, in contrast with WT, produces an interfascicular cambium and a large wood cylinder is the flowering stem. We present the RNAseq data for polyA mRNA of different developmental stages of cambium and wood formation in Arabidopsis thaliana. We sequenced 7 stages; 4 in the woody mutant soc1-6 ful-7 (herbaceous, cambium initiation, wood initiation and leaf) and 3 stages in the WT Col-0 (herbaceous, cambium and leaf). The corresponding stem anatomy is also presented in the manuscript indicating the stage of cambium development and the production of secondary xylem.

2016-05-21 | GSE69167 | GEO

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