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RNA-seq of bronchoalveolar lavage cells from wild-type mice reconstituted with wild-type or Cry1/2 double-knockout bone marrow.

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory lung disease characterized by alveolar destruction and impaired tissue repair. Although circadian disruption has been implicated in COPD pathogenesis, the underlying molecular mechanisms remain poorly understood. To examine the hematopoietic contribution of CRY1/2 to lung homeostasis, we generated bone-marrow–chimeric mice by retro-orbitally injecting 3.5 × 10⁶ bone marrow (BM) cells from Cry1/2 double-knockout (dKO) or age- and sex-matched wild-type (WT) donors into lethally irradiated CD45.1 recipient mice. Eight weeks after transplantation, bronchoalveolar lavage (BAL) cells were collected, and RNA-seq was performed to profile transcriptional changes driven by loss of Cry1/2 in the hematopoietic compartment.

ORGANISM(S): Mus musculus

PROVIDER: GSE313652 | GEO | 2026/07/30

REPOSITORIES: GEO

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