RNA-seq of WT and Rbm25-deficient peritoneal macrophages infected with vesicular stomatitis virus (VSV)

ABSTRACT: The purpose of this study is to detect differentially expressed genes in wild type (WT) and Rbm25-deficient peritoneal macrophages (PMs) infected with vesicular stomatitis virus for 4 hours. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Mouse PMs from WT and Rbm25-deficient mice were cultured in DMEM medium. PMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25-deficient PMs were infected with vesicular stomatitis virus for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina. We identified several differently expressed genes in the two groups of cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE314891 | GEO | 2026/07/15

REPOSITORIES: GEO