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Bulk RNA sequencing of interfollicular Tfh cells isolated from the palatine tonsils of recurrent tonsillitis and IgA nephropathy.

ABSTRACT: Immunoglobulin A nephropathy (IgAN) is a chronic glomerular disease characterized by mesangial IgA deposition, which can progress to end-stage renal failure. Tonsillectomy is used as a therapeutic intervention to slow disease progression and provide early clinical benefit. However, the immune mechanisms within the palatine tonsils that contribute to IgAN pathogenesis remain incompletely understood. To define the tonsillar immune environment that regulates pathological antibody production, we analyzed T cell subsets in relation to renal function. Interfollicular T follicular helper (IF-Tfh) cells (CD3+CD4+CD8-PD-1loCXCR5lo) were significantly expanded in IgAN tonsils compared with disease controls and strongly correlated with clinical markers of renal abnormalities. Transcriptomic analysis of IF-Tfh cells from IgAN tonsils revealed a distinct gene expression profile enriched for effector memory T cell–like features associated with kidney impairment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE315711 | GEO | 2026/02/13

REPOSITORIES: GEO

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