Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Temporal dynamics and functional annotation of transcriptome rhythmicity in HEK293T cells

ABSTRACT: HEK293T cells are a widely used experimental model in cell biology. However, the presence and robustness of intrinsic circadian rhythmicity in HEK293T cells remain controversial. To address this issue, we synchronized HEK293T cells with dexamethasone and initiated sample collection 24 hours after synchronization. Cells were harvested every 4 hours over a 48-hour period, followed by transcriptomic (RNA-seq) analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE315903 | GEO | 2026/03/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Temporal dynamics and functional annotation of transcriptome rhythmicity in HEK293T cells

Project description:Temporal dynamics and functional annotation of transcriptome rhythmicity in HEK293T cells

| PRJNA1399905 | ENA

HEK293T cells

Project description:HEK293T cells Raw sequence reads

| PRJNA905048 | ENA

HEK293T cells

Project description:HEK293T cells Raw sequence reads

| PRJNA948481 | ENA

Overexpression of USF1 in HEK293T cells

Project description:Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.

2009-09-14 | E-GEOD-17300 | biostudies-arrayexpress

ADAR2 in HEK293T cells

Project description:ADAR2 in HEK293T cells

| PRJNA422795 | ENA

Bidirectional histone monoaminylation dynamics regulate neural rhythmicity

Project description:H3 monoaminylation dynamics are bidirectionally controlled by TG2 and facilitate neural rhythmicity.

2024-10-10 | GSE270432 | GEO

Bidirectional histone monoaminylation dynamics regulate neural rhythmicity

Project description:H3 monoaminylation dynamics are bidirectionally controlled by TG2 and facilitate neural rhythmicity.

2024-10-10 | GSE270429 | GEO

Analysis of transcriptome of mouse melanoma cells co-cultured with HEK293T cells expressing mouse Siglec1.

Project description:To evaluate the effect of mouse Siglec1 on B16F10 melanoma we used co-culture methos where HEK293T cells were transiently transfected with plasmid containg mouse Siglec1 or empty plasmid (mock). These HEK293T cells were cultured with B16F10-GFP mouse melanoma cells for 18 hours in low binding plates. After 18 hours of incubation on 37°C, 5% CO2, melanoma cells were FACS sorted based on GFP expression and total RNA was isolated and used for TruSeq RNA library construction for transcriptome analysis. Samples were from 3 independent experiments. The library preparation and NGS data aquisition were performed by Macrogen (Macrogen, Inc., Korea)

2019-12-22 | GSE109077 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells Results: Differences in a set of microRNAs Conclusions: Nef-transfection induces changes in the microRNA content of exosomes

2018-11-18 | GSE122656 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells. Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells. Results: Differences in a set of mRNAs. Conclusions: Nef-transfection might induces changes in the mRNA content of exosomes.

2018-11-18 | GSE122655 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data