To screen the epigentic facters' effect on mESC state transition, Naïve state, Primed state.

ABSTRACT: The transcriptional and epigenetic status restricts the self-renewal ability and lineage specificity of the naïve and primed mouse embryonic stem cells (mESC). CRISPR/Cas9-based functional screening coupled with single cell RNA-seq (CROP-seq) establishes relationships between gRNA-mediated knockout genotypes and transcriptome phenotypes, and is a novel tool to unravel gene regulatory networks. Here, we used CROP-seq approach to dissect the transcriptional and epigenetic regulation of the pluripotency network in mESCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE316051 | GEO | 2026/08/19

REPOSITORIES: GEO