Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Longitudinal, Multi-omic Atlas Reveals the Emergence of a Spatially Organized Immunosuppressive Ecosystem in Resistant Melanoma

ABSTRACT: The goal of this study is to explore the mechanisms of resistance in melanoma under various immunotherapy treatments, particularly focusing on anti-PD-1 therapy, through the analysis of RPPA data. The study includes 38 tumor samples from two melanoma patients, with samples collected at pre-treatment, on-treatment, and post-treatment time points. These two patients were classified as non-responders (NRs). The RPPA data covers more than 300 antibodies targeting key signaling pathways, including hormone receptor signaling, HER family signaling, cell cycle/proliferation pathways, immune checkpoint regulation, DNA repair deficiency, AKT/mTOR/PI3K pathways, apoptosis/autophagy, IGF1R signaling, and RTK pathways. These data will serve as a valuable resource for validating resistance markers and understanding the signaling mechanisms driving tumor progression despite immune checkpoint blockade therapies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE317912 | GEO | 2026/01/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

protein levels in BRAF inhibitor resistance melanoma cells treated with PF3758309

Project description:11 BRAF inhibitor resistance melanoma cells were treated with PAK inhibitor PF3758309 for 48 hr, the cell lysis were analyzed by RPPA profiling by protein array (RPPA)

2017-07-31 | GSE96753 | GEO

protein levels in BRAF/MEK inhibitor resistance melanoma cells treated with PF3758309 [CR]

Project description:3 BRAF/MEK inhibitor resistance melanoma cells were treated with PAK inhibitor PF3758309 for 48 hr, the cell lysis were analyzed by RPPA profiling by protein array (RPPA)

2017-07-31 | GSE96902 | GEO

Interleukin enhancer-binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma

Project description:This study aimed to understand the role of ILF2 upregulation in metastatic melanoma cutaneous progression and DNA damage response. The goal of RPPA analysis was to determine the protein expression profiles in DP-0574 and IM-0223 melanoma cell lines with ILF2 overexpression (ILF2-OV) or control empty vector (EV). By analysis of RPPA in both metastatic melanoma cell lines, we found that ILF2-OV controls significantly increased RAD50 expression.

2022-04-15 | GSE159692 | GEO

DNA Methylation Inhibitors in Immunotherapy

Project description:<p>We show that DNA methyltransferase inhibitors (DNMTis) upregulate immune signaling in cancer through the viral defense pathway and re-expression of epigenetically silenced endogenous retrovirus. In melanoma patients treated with an immune checkpoint therapy, high viral defense signature expression in tumors significantly associates with durable clinical response and DNMTi treatment sensitizes to anti-CTLA4 therapy in a pre-clinical melanoma model.</p>

| phs001038 | dbGaP

Preactivation of the interferon signaling in liver is correlated with non-response to interferon alpha therapy in patients chronically infected with hepatitis B virus

Project description:To screen the differentially expressed genes between responders(Rs) and nonresponders(NRs) in CHB patients treated with IFN-α, and to explore the molecular basis for treatment failure.Expression profiling was performed on percutaneous needle liver biopsy specimens taken before therapy.Gene expression levels were compared between 7 NRs and 6 Rs,Gene Ontology category and KEGG pathway were analyzed for differentially expressed genes, and the selected differentially expressed genes were confirmed using real-time polymerase chain reaction.NRs and Rs have different liver gene expression profiles before IFN-α treatment. Preactivation of the IFN signaling pathway leading to the increased expression of inhibitory ISGs and inhibition of immune response in the pretreatment livers were associated with NRs. Expression profiling was performed on percutaneous needle liver biopsy specimens taken before therapy.Gene expression levels were compared between 7 NRs and 6 Rs. This dataset is part of the TransQST collection.

2011-03-01 | E-GEOD-27555 | biostudies-arrayexpress

I-SPY2-990 mRNA/RPPA Data Resource: RPPA component

Project description:The RPPA component of the I-SPY2-990 mRNA/RPPA Data Resource contains protein/phosphoprotein data from pre-treatment laser capture microdissected (LCM) tumor biopsies for 139 key signaling proteins/phosphoproteins in cancer for 736 patients from 8 arms of the neoadjuvant I-SPY2 TRIAL (NCT01042379) for aggressive early stage breast cancer [194 Control (Ctr); 63 veliparib/carboplatin (VC); 105 neratinib (N); 87 MK2206; 128 trebananib; 49 TDM1/pertuzumab(P); 43 H/P; and 67 pembrolizumab (pembro)]. This record also contains clinical data for these patients, including HR, HER2 and MP status, response (pCR or no pCR), and treatment arm. RPPA endpoints assayed are from hormone receptor (n=4), HER family (n=14), cell cycle/proliferation (n=20), immune (n=18), DNA repair deficiency (DDR; n=15), AKT/mTOR/PI3K (n=7), apoptosis/autophagy (n=10), IGF1R (n=6), TIE/ANG (n=4), growth/survival/metabolism (n=22) and RTK (n=19) pathways. For gene expresson data for all 736 patients with RPPA data, plus an additional 150 patients for a total 987 patients from 10 arms of I-SPY2 (mRNA component of the I-SPY2-990 Data Resource), see the companion GEO record/subseries GSE194040.

2022-05-25 | GSE196093 | GEO

Comparative analysis of gene expression in tumors responding and non-responding to anti-CTLA-4 in a preclinical study of melanoma

Project description:To investigate the diverse responses of the M4 mouse melanoma to immune checkpoint inhibitors, the tumors responding (R) and nonresponding (NR) to anti-CTLA-4 from a preclinical study of M4 melanoma were subjected to mRNA sequencing. The differentially expressed genes were analyzed to identify the signaling pathways that asscoiated with the therapeutic responses of the tumors.

2024-02-29 | GSE255484 | GEO

Preactivation of the interferon signaling in liver is correlated with non-response to interferon alpha therapy in patients chronically infected with hepatitis B virus

2011-03-01 | GSE27555 | GEO