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A Wnt7a-Hoxa13 pathway for Müllerian duct fusion at the body midline for uterine morphogenesis

ABSTRACT: During embryonic development, the Müllerian ducts and adjacent mesenchyme differentiate into the tissues of the oviducts, uterus, cervix, and upper vagina. Variations in the fusion process can alter typical uterine morphology, correlating with an increased incidence of infertility, high-risk pregnancy, and miscarriage. We show that Wnt7a is required for Müllerian duct fusion at the body midline to generate the bipartite uterus of the mouse. Wnt7a null female mice form Müllerian ducts, but the ducts do not fuse at the midline resulting in a duplex reproductive tract (two separate uterine horns, two cervices, and two vaginas). Spatial transcriptome analysis of Wnt7a null and control fusion regions identified a downregulation of Hoxa13 in Wnt7a-null mesenchyme compared to wild type. Reduction in Hoxa13/HOXA13 is associated with didelphic uterine formation. These studies identify a Wnt7a-Hoxa13 genetic pathway that regulates uterine morphology.

ORGANISM(S): Mus musculus

PROVIDER: GSE318449 | GEO | 2026/02/07

REPOSITORIES: GEO

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A Wnt7a-Hoxa13 pathway for Müllerian duct fusion at the body midline for uterine morphogenesis

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