Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

New Insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo

ABSTRACT: In vertebrate embryos, Müllerian ducts give rise to the female reproductive tract. Aberrations in duct development cause reproductive disorders, many of which have an unknown etiology. Here, we present a comprehensive analysis of Müllerian duct differentiation using single cell transcriptomics in the chicken embryo, a unique model for duct development. The data reveal greater cellular complexity of Müllerian duct differentiation than previously understood. Duct mesenchyme has two transcriptionally distinct populations, while duct epithelium shows distinct regionalised compartments. Trajectory analysis shows that these populations have different fates. Single cell transcript profiling of the regressing male mesenchyme identifies new genes implicated in duct regression. One of these male-restricted transcripts, DKK4, responds to Anti-Mullerian Hormone induction and can induce duct regression when ectopically expressed in females. Lastly, the right female duct does not regress in the same manner as in males, being transcriptionally distinct. These data provide new insights into Müllerian duct differentiation.

ORGANISM(S): Gallus gallus

PROVIDER: GSE323377 | GEO | 2026/04/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome and chromatin accessiblity analysis of Gli1+ and Amhr2+ mesenchymes in the fetal murine reproductive tracts

Project description:Precursors for the male and female reproductive tracts (also known as the Wolffian duct and the Müllerian duct) co-exist in a mammalian embryo before sexual differentiation. The Wolffian duct and Müllerian duct are surrounded by their own mesenchymes in the mesonephros, which can be distinguished by their specific expression of Gli1 and Amhr2, respectively. In the XX embryo, during sexual differentiation, the Wolffian duct degenerates. In this study, by tracking the fate of the Gli1+ Wolffian duct mesenchyme in the tamoxifen inducible genetic lineage tracing model Gli1-CreER; Rosa-Tomato, we demonstrate that the Wolffian duct mesenchyme does not regress along with the Wolffian duct epithelium in the XX embryo. Instead, the Wolffian duct mesenchyme is repurposed and becomes a unique mesenchymal population in the female reproductive tract. Profiling the transcriptomes and chromatin accessibilities of the Gli1+ Wolffian duct mesenchyme in XX and XY during sexual differentiation (E14.5 and E16.5) shows signaling pathways and transcriptional factors that potentially involves the female and male fate differentiation of the Gli1+ Wolffian duct mesenchyme. Comparing the transcriptomes between the Gli1+ Wolffian duct mesenchyme and the Amhr2+ Müllerian duct mesenchyme from the neonatal uterus reveals similarities and differences between the two mesenchymal populations in the female reproductive tract. Ex vivo ablation of the Wolffian duct mesenchyme stunted the growth of the fetal female reproductive tract. In summary, our discovery of the contribution of the Wolffian duct mesenchyme to the female reproductive tract provides new insights into our understanding of sexual differentiation of reproductive tracts.

2022-10-05 | GSE179876 | GEO

Müllerian duct maintenance at the cranial region is promoted by Gata2

Project description:The paradigm of sexual differentiation holds that female embryos retain Müllerian ducts (the precursor of female reproductive tract organs) by default in the absence of anti-Müllerian hormone (AMH). Whether Müllerian duct maintenance requires active signaling has remained unclear. Here, we show that deletion of mesenchymal Gata2 induces selective regression of the cranial Müllerian duct (future oviduct). This regression did not result from ectopic AMH signaling but from loss of region-specific NRG1 signaling. In contrast, the caudal Müllerian duct (future uterus) was retained but displayed disrupted epithelial differentiation when Gata2 was deleted in either mesenchymal or epithelial compartments. Our findings reveal a region-specific, GATA2-dependent program that actively maintains the cranial Müllerian duct, reshaping our understanding of female reproductive tract development.

2026-03-16 | GSE324404 | GEO

Osterix functions downstream of anti-Müllerian hormone signaling to regulate Müllerian duct regression

Project description:The goal of this study was to identify potential AMH-induced genes and regulatory networks controlling regression by RNA-Seq transcriptome analysis of differences in Müllerian Duct mesenchyme between males (AMH signaling on) and females (AMH signaling off) in purified fetal Müllerian Duct mesenchymal cells. This analysis found 82 genes up-regulated in males during MD regression and identified Osterix (Osx)/Sp7, a key transcriptional regulator of osteoblast differentiation and bone formation, as a novel downstream effector of AMH signaling during MD regression.

2018-07-16 | GSE116157 | GEO

A Wnt7a-Hoxa13 pathway for Müllerian duct fusion at the body midline for uterine morphogenesis

Project description:During embryonic development, the Müllerian ducts and adjacent mesenchyme differentiate into the tissues of the oviducts, uterus, cervix, and upper vagina. Variations in the fusion process can alter typical uterine morphology, correlating with an increased incidence of infertility, high-risk pregnancy, and miscarriage. We show that Wnt7a is required for Müllerian duct fusion at the body midline to generate the bipartite uterus of the mouse. Wnt7a null female mice form Müllerian ducts, but the ducts do not fuse at the midline resulting in a duplex reproductive tract (two separate uterine horns, two cervices, and two vaginas). Spatial transcriptome analysis of Wnt7a null and control fusion regions identified a downregulation of Hoxa13 in Wnt7a-null mesenchyme compared to wild type. Reduction in Hoxa13/HOXA13 is associated with didelphic uterine formation. These studies identify a Wnt7a-Hoxa13 genetic pathway that regulates uterine morphology.

2026-02-07 | GSE318449 | GEO

Expression data from E14.5 mouse agonadal urogential ridges

Project description:We analyzed global mRNA expression in urogenital ridges of embryos that were either heterozygous for the Müllerian Inhibiting Substance type II receptor or homozygously deleted to study the genes regulated by MIS during Müllerian duct regression.

2013-05-01 | GSE38009 | GEO

Genome-wide binding sites of COUP-TFII in the E14.5 mesonephroi

Project description:In an XX embryo, the Wolffian duct regresses under the control of the mesenchymal transcription factor COUP-TFII. To understand cellular and molecular actions underlying Wolffian duct regression, we preformed transcriptomic analyses of XX mesonephroi with or without Coup-tfII and genome-wide analysis of COUP-TFII chromatin occupancy in XX mesonephroi. The integrative analysis of COUP-TFII genome-wide binding and transcriptomic analysis revealed the suppression of muscle differentiation and extracellular matrix genes by COUP-TFII and identified a group of COUP-TFII’s potential transcriptional partners in the mesenchyme that potentially facilitate Wolffian duct regression. These findings provide insights into the molecular action of COUP-TFII in the Wolffian duct mesenchyme and identify a list of biologically relevant candidate genes and pathways for future functional analyses in sexual differentiation of reproductive tracts.

2022-04-20 | GSE157836 | GEO

Single-cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Project description:The upper Müllerian duct (MD) is patterned and specified into two morphologically and functionally distinct organs, the oviduct and uterus. It is known that this regionalization process is instructed by inductive signals from the adjacent mesenchyme. However, the interaction landscape between epithelium and mesenchyme during upper MD development remains largely unknown. Here, we performed single-cell transcriptomic profiling of mouse neonatal oviducts and uteri at the initiation of MD epithelial differentiation (postnatal day 3). We identified major cell types including epithelium, mesenchyme, pericytes, mesothelium, endothelium, and immune cells in both organs with established markers. Moreover, we uncovered region-specific epithelial and mesenchymal subpopulations and then deduced region-specific ligand-receptor pairs mediating mesenchymal-epithelial interactions along the craniocaudal axis. Unexpectedly, we discovered a mesenchymal subpopulation marked by neurofilaments with specific localizations at the mesometrial pole of both the neonatal oviduct and uterus. Lastly, we analyzed and revealed organ-specific signature genes of pericytes and mesothelial cells. Taken together, our study enriches our knowledge of upper Müllerian duct development, and provides a manageable list of potential genes, pathways, and region-specific cell subtypes for future functional studies.

2023-12-21 | GSE251724 | GEO

RNA Sequencing of ARK2 PAX8 Knock Down Cells

Project description:PAX8 is an important marker of the Mullerian duct and is often overexpressed in endometrial cancers. We sought to understand what PAX* is regulating by knocking down its expression and performing RNA-sequencing.

2024-09-13 | GSE260631 | GEO

Transcriptional landscape of the embryonic chicken Müllerian duct

Project description:We report here high-throughput RNA sequencing (RNA-seq) to characterise the transcriptional landscape of the embryonic chicken Müllerian duct during development.

2020-10-07 | GSE153029 | GEO

New Insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo

Project description:New Insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo

| PRJNA1432706 | ENA