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Spatial transcriptomics data of the wild-type and App NL-G-F mouse posterior cortex

ABSTRACT: Our in vivo imaging data suggest that GABAergic microcircuitry is dysfunctional in the App NL-G-F mouse. To determine the relative contribution of different GABAergic subclasses to abnormal synaptic processing, we tested for cell-type-specific molecular processes associated with early-stage amyloidosis. To do this, we used the CosMx (Nanostring/Bruker) spatial transcriptomic system to investigate cellular changes in posterior cortical regions (visual and retrosplenial cortices) identified as expressing dysregulated resting-state activity in 3-4 months and 6-8 months App NL-G-F mice. Our results suggest that parvalbumin interneurons show some of the earliest transcriptomic changes in posterior cortical regions, with dysregulated activity in App NL-G-F mice, which are linked to a downregulation of GABAergic synaptic transmission. Both parvalbumin and somatostatin interneurons show the highest number of transcripts that covary with local plaque load, which are associated with synaptic processes.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE318590 | GEO | 2026/02/11

REPOSITORIES: GEO

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