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High-Throughput Promoter Strength Sequencing Source Data

ABSTRACT: This study was designed to construct and quantitatively characterize a synthetic promoter library in Escherichia coli. Promoter variants were assembled into the low-to-medium copy plasmid pSEVA221 to generate the p221-Plib library, in which each promoter was associated with a barcode sequence. The library was introduced into E. coli DH5α, and pooled transformants were cultured under selective conditions. Promoter activities were determined by sequencing barcode abundances from both RNA-derived cDNA and plasmid DNA libraries. The normalized RNA/DNA ratio was used as a quantitative measure of promoter activity, correcting for differences in sequencing depth, plasmid abundance, and clone representation. This dataset provides promoter activity profiles that can facilitate promoter selection and optimization for synthetic biology and metabolic engineering applications.

ORGANISM(S): Escherichia coli DH5[alpha]

PROVIDER: GSE318900 | GEO | 2026/02/16

REPOSITORIES: GEO

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