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Single-cell profiling identifies glial transcriptional remodeling in the trigeminal ganglion after corneal injury

ABSTRACT: The cornea depends on trigeminal sensory innervation for sensation and epithelial homeostasis, yet cell type–specific transcriptional responses in the trigeminal ganglion following corneal injury remain incompletely defined. To address this gap, we combined bulk and single-cell RNA sequencing to profile the mouse trigeminal ganglion after a moderate corneal alkali injury associated with transient denervation and partial reinnervation. Bulk transcriptomic analysis defined a dominant injury-associated axis enriched for protein folding and immune-related pathways. Single-cell profiling resolved these changes across neuronal and non-neuronal populations, revealing distinct transcriptional programs in neurons, Schwann cells, and satellite glial cells, including coordinated modulation of metabolic, immune, and proteostasis-related pathways. In parallel, inference of ligand–receptor interactions identified injury-associated reorganization of predicted intercellular communication networks over time. Together, these data define a corneal injury–associated transcriptional landscape of the trigeminal ganglion, highlighting cell type–specific engagement of proteostasis and stress-related programs across neuronal and glial populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE322671 | GEO | 2026/09/02

REPOSITORIES: GEO

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