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Effects of Rg3@PACVs with NIR in a Rat Model of Pulmonary Ischemia–Reperfusion Injury

ABSTRACT: Pulmonary ischemia–reperfusion injury (IRI) is characterized by excessive inflammation, oxidative stress, and mitochondrial dysfunction, leading to severe lung damage. To elucidate the molecular mechanisms underlying the protective effects of a macrophage membrane-coated mesoporous polydopamine nanoparticle system loaded with Ginsenoside Rg3 (Rg3@PACVs) combined with near-infrared (NIR) irradiation, we performed transcriptome sequencing of rat lung tissues. Differential gene expression profiles were compared between IRI and IRI + Rg3@PACVs + NIR groups. A total of 209 upregulated and 332 downregulated genes were identified in the treatment group relative to IRI controls. Functional enrichment analyses revealed that Rg3@PACVs + NIR pretreatment enhanced pathways related to endoplasmic reticulum stress response, PI3K-AKT signaling, oxygen transport, protein folding, hypoxia response, TCA cycle activity, ATP-dependent processes, and cell cycle progression. In contrast, inflammation-associated pathways including TLR/NF-κB signaling, cytokine binding, cell adhesion molecules, neutrophil extracellular trap formation, IL-1β production, and leukocyte trans-endothelial migration were significantly suppressed. GSEA further demonstrated reduced oxidative stress (cytochrome P450 activity) and enhanced FOXO signaling. These findings indicate that Rg3@PACVs + NIR mitigates pulmonary IRI by coordinately suppressing inflammatory responses, alleviating oxidative stress, and restoring mitochondrial homeostasis, thereby promoting lung tissue repair.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE324247 | GEO | 2026/03/10

REPOSITORIES: GEO

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