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WTAP tetramer ensures m⁶A writer assembly and faithful mitosis

ABSTRACT: The m6A methyltransferase complex (“writer”) regulates mRNA stability and translation, but how its assembly is orchestrated remains incompletely understood. Wilms’ tumor 1-associating protein (WTAP) is a conserved regulatory subunit essential for m⁶A deposition and cell proliferation, yet its structural organization and mechanistic contributions remain elusive.Our live-cell imaging demonstrates that WTAP is required for accurate chromosome segregation, and MeRIP-seq analysis identifies WTAP-dependent m⁶A regulation as a critical determinant sustaining the expression of mitotic regulators, including KIF20A. Together, our study defines a tetrameric scaffold function for WTAP that is essential for writer complex integrity and highlights its pivotal role in linking m⁶A methylation to cell cycle progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE324500 | GEO | 2026/03/23

REPOSITORIES: GEO

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