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Dextran-coated Iron Oxide Nanoparticles impairs cardiac function in zebrafish larvae

ABSTRACT: Iron Oxide Nanoparticles (IONPs) with no doubt are the next platform for theragnostic in the biomedical field. Cardiovascular pathologies take immense advantage from the use of these nanoparticles in vascular imaging, detection of inflammatory and atherosclerotic lesions and magnetic therapies. However, the potential toxicity caused by the iron ions released from the IONPs core and its underlying mechanism is still unclear. Iron overload in the heart is known to cause disruption in calcium, sodium, and potassium ion channels, arrhythmias, and diastolic and systolic dysfunction. In this study, we employed a toxicity protocol to examine the developmental toxicity and cardiac effects of exposure on a zebrafish larva to Dextran coated IONPs. RNAseq analysis was conducted to establish the mechanisms of toxicity in Tg(fli:EGFP) fish. We validate the results using a series of embryonic cardiovascular function and a vascular development assay. Our results showed a developmental delay in the Dx-IONPs group, pronounced specially in vascular growth, visible in Tg(fli:EGFP) fish. Cardiac function analysis allowed to establish a correlation between Dx-IONP group and Iron salt exposed group from RNAseq. This work brings to light not only phenotypical, but also molecular basis for a better understanding of IONPs toxicity.

ORGANISM(S): Danio rerio

PROVIDER: GSE325727 | GEO | 2026/03/26

REPOSITORIES: GEO

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