The stability of m6A-marked transcripts is linked to cell identity in planarians [MTC KD]

ABSTRACT: Using Nanopore dRNA-Seq, we generated a high-confidence atlas of ~72,200 m6A sites (minimal methylation rate of 15% in at least two replicates) across the planarian transcriptome. We identified planarian m6A sites to be installed in a DRAYW context and to be highly enriched near stop codons. Upon depletion of METTL14 – a core component of the methyltransferase complex – we observed a significant reduction of m6A levels by 73.2%, as well as significant gene expression changes in 1059 genes. Loss of m6A resulted in up- as well as downregulation of genes, but cell type markers were found to be specifically represented in both gene classes.

ORGANISM(S): Schmidtea mediterranea

PROVIDER: GSE325806 | GEO | 2026/08/18

REPOSITORIES: GEO