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Single-cell short- and long-read transcriptomic atlas of seven reproductive-axis tissues in male Baoshan pig

ABSTRACT: Single-cell transcriptomic data were generated from seven reproductive-axis tissues of an 18-month-old male Baoshan pig, including hypothalamus, pituitary, prostate, bulbourethral gland, seminal vesicle, epididymis, and testis. To characterize both gene-expression profiles and transcript isoform diversity across these tissues, single-cell short-read libraries were sequenced on an Illumina platform and single-cell long-read libraries were sequenced on an Oxford Nanopore platform. Short-read data were processed with Cell Ranger, whereas long-read data were processed with FLAMES and further assessed for isoform structure. The dataset provides cell-level gene expression matrices, transcript/isoform-level quantification, isoform annotation files, and accompanying cell metadata. These data may facilitate studies of cellular heterogeneity and transcript isoform usage across the porcine reproductive axis.

ORGANISM(S): Sus scrofa

PROVIDER: GSE326370 | GEO | 2026/07/04

REPOSITORIES: GEO

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