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ScRNA-seq of limb-mesenhymal organoid derived from mouse embryo during digit-like morphogenesis

ABSTRACT: We performed single-cell RNA sequencing of mouse embryonic limb mesenchymal cells and limb mesenchymal organoids during digit-like morphogenesis. Distal and proximal limb mesenchymal cells were isolated from E11.5 Slc:ICR mouse embryos, labeled using lipid-DNA CellPlex/Feature Barcode oligonucleotides, and profiled either immediately as Day0 samples or after culture as limb mesenchymal organoids under serum-only control or Fgf8b/Wnt3a-supplemented conditions. The dataset includes Day0 distal and proximal cells, Day1 and Day2 control organoids, and Day1 and Day2 Fgf8b/Wnt3a-treated organoids. These data were used to characterize distal and proximal cell states, treatment-dependent transcriptional changes, and lineage/state trajectories associated with self-organized digit-like morphogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE331455 | GEO | 2026/05/24

REPOSITORIES: GEO

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