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Transcriptomic analysis of (S)-1,3-butanediol degradation pathway in Pseudomonas putida KT2440

ABSTRACT: (S)-1,3-butanediol [(S)-1,3-BDO] is a chiral diol and stereoisomer of (R)-1,3-BDO with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (S)-1,3-BDO and (S)-3-hydroxybutyrate [(S)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Differential gene expression analysis was performed to identify genes specifically induced during (S)-1,3-BDO and (S)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.

ORGANISM(S): Pseudomonas putida KT2440

PROVIDER: GSE333646 | GEO | 2026/06/01

REPOSITORIES: GEO

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