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RNA-seq analysis reveals transcriptional disruption induced by combined Cisplatin and TiO₂ nanoparticles in Drosophila melanogaster

ABSTRACT: Cisplatin, the first metal-based chemotherapeutic drug, remains widely used despite its toxicity and the emergence of drug resistance. Combining Cisplatin with nanoparticles has been proposed to improve its therapeutic profile, although most studies rely on in vitro models. Here, we investigated the in vivo transcriptional effects of Cisplatin (50 μg/ml) and titanium dioxide nanoparticles (TiO₂ NPs; 50 μg/ml), alone and in combination, in Drosophila melanogaster. Using RNA-seq and bioinformatic analyses, we assessed differential gene expression across treatments. Flies exposed to Cisplatin or TiO₂ NPs alone exhibited modulation of genes associated with xenobiotic metabolism and detoxification pathways. In contrast, combined exposure resulted in a markedly reduced number of differentially expressed genes (four DEGs), accompanied by disruption of pathways related to cell division and DNA/RNA metabolism. Mortality rates were not significantly affected in any group. These results suggest that co-exposure to Cisplatin and TiO₂ nanoparticles induces a global transcriptional suppression, potentially impairing essential cellular processes.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE334628 | GEO | 2026/07/07

REPOSITORIES: GEO

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