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Transcriptional response of A549 lung cancer cells to X-rays under normoxia, or chronic or transient hypoxia

ABSTRACT: The aim of this project was to study the X-ray-induced changes in A549 on the gene expression level under different oxygen conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE334631 | GEO | 2026/06/24

REPOSITORIES: GEO

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Transcriptional response of A549 lung cancer cells to carbon ions under normoxia, or chronic or transient hypoxia

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RNA-sequencing in irradiated and normal A549 cells.

Project description:The aim of this study is to investigate the role of circRNAs/miRNAs/lncRNAs in radioresistance in A549 cells. For irradiation, A549 cells were exposed to a single fraction 2 GY or 4GY X-ray dose using an X-RAD 160-225 instrument (Precision X-Ray, Inc., Branford, CT, USA; filter: 2 mm AI; 42 cm, 225 kV/s, 12.4 mA, 2.0 Gy/min). The irradiated A549 cells (after a single dose of irradiation) and normal A549 cells were cultured for further 48h and then collected its total RNA for high-throughput sequencing.

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