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3' end sequencing of HeLa-NXF1-dTAG cells upon NXF1 degradation to characterize alternative polyadenylation dynamics

ABSTRACT: Nuclear export factor 1 (NXF1) is the principal mRNA export receptor in eukaryotes. To characterize the role of NXF1 in regulating alternative polyadenylation (APA) dynamics, we employed the dTAG degradation system in a HeLa-NXF1-dTAG stable cell line. Cells were treated with 500 nM dTAG-13 or DMSO (vehicle control) for 6 hours to induce acute NXF1 depletion. Total RNA was subjected to 3' end sequencing (QuantSeq 3' mRNA-Seq Library Prep Kit V2, Lexogen) for APA analysis. Each condition was performed in biological duplicate.

ORGANISM(S): Homo sapiens

PROVIDER: GSE334799 | GEO | 2026/06/14

REPOSITORIES: GEO

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The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation and mRNA export

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