Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell transcriptomic profiling of 4-NQO-induced oral squamous cell carcinoma in a mouse model

ABSTRACT: To investigate the cellular heterogeneity and molecular drivers of oral squamous cell carcinoma (OSCC), we performed single-cell RNA sequencing (scRNA-seq) on tongue tissues from a 4-nitroquinoline 1-oxide (4-NQO)-induced mouse model. This dataset revealed eight distinct cell populations, with a significant reduction in epithelial cells and an increase in fibroblasts in OSCC compared to controls. Notably, a distinct Col1a1⁺ epithelial subpopulation was identified that exhibited the highest epithelial-mesenchymal transition (EMT) signature and was significantly expanded in OSCC. This study provides a valuable resource for understanding the transcriptional landscape and cellular dynamics underlying OSCC progression.

ORGANISM(S): Mus musculus

PROVIDER: GSE335490 | GEO | 2026/06/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide tongue epithelia transcriptomic profiles from K14-rtTA;TRE-FLBmi-1 + 4-nitroquinoline 1-oxide (4-NQO), 25 weeks (KrTB-N (25w)), KrTB+Doxycycline+4-NQO, 4 weeks (KrTB-DN (4w)), and KrTB+Doxycycline+4-NQO, 10 weeks (KrTB-DN (10w)).

Project description:We developed a transgenic mouse model (truncated K14-rtTA; TRE/Bmi1, KrTB) containing a doxycycline- (dox) controlled, Tet-responsive element system to selectively overexpress BMI1 only in the basal epithelial stem cells (SCs) of the tongue. Here we used this transgenic mouse line to delineate BMI1 actions in early-stage oral squamous cell carcinoma (OSCC). We observed more oncogenic changes in mice with ectopic BMI1 expression after 4 weeks of 4-nitroquinoline 1-oxide (4-NQO) treatment. We detected increased proliferation and oxidative stress, as well as increased expression of multiple transcripts and proteins linked to human OSCCs, in murine tongue epithelia with high BMI1 expression during early carcinogen-induced tumorigenesis. Furthermore, increases in mRNAs encoding multiple metabolic targets, such as SLC16A3, PKM, and GPI1, were accelerated upon BMI1 overexpression in our 4-NQO model.

2025-12-09 | GSE307391 | GEO

Genome-wide tongue epithelia transcriptomic profiles from K14-rtTA;TRE-FLBmi-1 (KrTB), KrTB+Doxycycline (KrTB-D), KrTB+4-nitroquinoline 1-oxide (KrTB-N), and KrTB+Doxycycline+4-nitroquinoline 1-oxide (KrTB-DN).

Project description:We developed a transgenic mouse model (truncated K14-rtTA; TRE/Bmi1, KrTB) containing a doxycycline- (dox) controlled, Tet-responsive element system to selectively overexpress BMI1 only in the basal epithelial SCs of the tongue. Here, we used this model to delineate BMI1 actions in tongue epithelia during oral tumorigenesis (as induced by 4-nitroquinoline 1-oxide, 4-NQO). Genome-wide transcriptomics indicated that mRNAs associated with human OSCC, including SOX9, HIF1A, MMP9, INHBB, and MYOF, were further increased by ectopic BMI1 expression in murine tongue epithelia. mRNAs encoding multiple metabolic targets, such as SLC2A1 (GLUT1), PKM, LDHA, and HK2, were also increased upon BMI1 overexpression in 4-NQO-treated tongue epithelia.

2025-03-19 | GSE285101 | GEO

Transcription profiling of mouse lymphoma L5178Y cells treated with 4-NitroQuinoline N-Oxide (4-NQO) ILSI-HESI Genotoxicity Study - 4-NQO

Project description:Mouse lymphoma L5178Y cells are treated with 4-NitroQuinoline N-Oxide (4-NQO) [CAS:56-57-5;CHEBI:16907] and harvested at 4 and 24 hours for analysis.

2006-02-13 | E-TOXM-1 | biostudies-arrayexpress

Genome-wide changes in transcript levels in tongues after 4-NQO and ethanol treatment

Project description:Tobacco use and alcohol consumption are two major contributing factors for head and neck squamous cell carcinoma (HNSCC) carcinogenesis. We combined the 4-nitroquinoline-1-oxide (4-NQO) oral carcinogenesis mouse model and the Meadows-Cook alcohol mouse models and performed next generation genome-wide RNA-sequencing of tongues. We determined changes in transcript levels in four groups: 4-NQO followed by ethanol treatment (4-NQO/EtOH), 4-NQO followed by normal drinking water (4-NQO/Untr.), vehicle control followed by ethanol treatment (V.C./EtOH), and vehicle control followed by normal drinking water (V.C./Untr.). We found that the 494 gene transcripts were significantly changed (at least a 2-fold change where p<0.05) in the V.C./EtOH group compared to the V.C./Untr. group. The 4-NQO/Untr. group had 1,808 transcripts significantly changed compared to the V.C./Untr group, while the 4-NQO/EtOH group had 3,606 significantly changed transcripts as compared to the V.C/Untr. group. This study is the first to show that 4-NQO followed by ethanol cause the largest number of changes in transcript levels in the tongue.

2015-10-06 | GSE62125 | GEO

Gene expression of mouse oral cancer cell lines

Project description:We established two murine OSCC cell lines, named M1-2 and M2-3, from a mouse tumor induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline. After in vitro selection using sphere culture, NHRI-HN1 and NHRI-HN2 were derived from M1-2 and M2-3 cells, respectively. Only NHRI-HN1 cells are capable of generating orthotopic tumors in syngeneic mice.

2022-09-21 | GSE139282 | GEO

Genome-wide analysis of gene expression changes in mouse tongue squamous cell carcinomas induced by 4-nitroquinoline 1-oxide (4-NQO)

Project description:We hypothesized that the expression of many genes are dysregulated during oral cancer carcinogenesis. We examined genome-wide transcript levels in normal mouse tongues and the tongue squamous cell carcinomas induced by 4-NQO. The results will provide important information for the diagnosis, prevention, and treatment of human oral cancers, including tongue cancer. Total RNA obtained from normal tongues (not treated with 4-NQO) and tongue squamous cell carcinomas induced by 4-NQO.

2014-07-01 | E-GEOD-54246 | biostudies-arrayexpress

Genomic and transcriptomic landscape of a mouse model for immunotherapy in oral squamous cell carcinoma

Project description:We examined the 4-nitroquinoline-1-oxide (4NQO)-induced oral squamous cell carcinoma (OSCC) model for genetic aberrations, transcriptomic profile, and immune cell compositions at different pathological stages.

2023-08-01 | GSE229289 | GEO

MPA and NQO studies (Sevilla)

Project description:RNA polymerases deal with obstacles during transcription elongation that need to be removed. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TCR). To improve our knowledge of transcription elongation and its coupling to TCR, we performed DNA microarray experiments and used the yeast library of nonessential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor micophenolic acid and the DNA-damaging agent 4- nitroquinoline-N-oxide. A single sample for each treatment was analyzed.

2008-12-29 | E-GEOD-11561 | biostudies-arrayexpress

Genome-wide analysis of gene expression changes in mouse tongue squamous cell carcinomas induced by 4-nitroquinoline 1-oxide (4-NQO)

2014-07-01 | GSE54246 | GEO

MPA and NQO studies (Sevilla)

2008-12-30 | GSE11561 | GEO