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DCas9-KRAB CRISPRi-mediated transcriptional repression of TERT in H1299 non-small cell lung cancer cells

ABSTRACT: Bulk RNA sequencing was performed to assess transcriptome-wide changes associated with dCas9-KRAB mediated CRISPR interference (CRISPRi) of telomerase reverse transcriptase (TERT) in H1299 non-small cell lung cancer cells. H1299 cells were transduced with a lentiviral vector co-expressing dCas9-KRAB and either no guide RNA (non-targeting control) or a TERT-promoter-targeting single-guide RNA, selected with puromycin, and harvested at day 33 after transduction. Differential expression analysis was used to evaluate the specificity of sustained TERT repression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE336428 | GEO | 2026/06/24

REPOSITORIES: GEO

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