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FastGro-seq: POLR3G and POLR3GL knockout and HEK293T cell treated with Pol III inhibitor ML60218

ABSTRACT: RNA polymerase III (Pol III) synthesizes noncoding RNAs essential for translation, RNA processing, and transcription regulation. Its activity aligns with cellular growth and nutrient availability, and its dysregulation is linked to diseases like breast cancer and leukodystrophy. RNA polymerase III composition is shaped by the mutually exclusive incorporation of two paralogous subunits, RPC7α and RPC7β, encoded by genes POLR3G and POLR3GL in vertebrates. The expression of POLR3G and POLR3GL is spatiotemporally regulated during development, and multiple reports point to RPC7α-enhanced Pol III activity patterns, indicating that Pol III identity may underlie dynamic Pol III transcription patterns observed in higher eukaryotes. In cancer, upregulation of POLR3G, but not POLR3GL, is associated with poor survival outcomes among patients, suggesting differences between RPC7α and RPC7β further influence disease progression and may translate into future biomarkers and therapeutic strategies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE337522 | GEO | 2026/07/10

REPOSITORIES: GEO

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small RNA seq: POLR3G and POLR3GL knockout in THP-1 and POLR3G knockdown in HEK293T

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