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HOXA3 regulates thymic epithelial cell differentiation from hESCs and supports T-cell differentiation through CXCL12

ABSTRACT: H9 human embryonic stem cells were differentiated into thymic epithelial cells (TECs) via a three-stage protocol. HOXA3 was knocked down during the 3PPE-to-TEC transition to investigate its role in TEC differentiation and function. RNA-seq was performed on HOXA3 knockdown and control TEC-like cells (3 biological replicates each). HOXA3 knockdown disrupted epithelial differentiation and reduced TEC-associated gene expression, including K8, AIRE, and CD205, along with decreased FOXN1 protein. HOXA3-deficient TECs showed impaired support for T-cell generation from CD34+ progenitors. CXCL12 was identified as a downregulated gene and confirmed at mRNA and protein levels. Exogenous CXCL12 partially rescued the impaired T-cell-supporting function. This dataset provides insights into molecular regulation of human TEC differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE344232 | GEO | 2026/08/24

REPOSITORIES: GEO

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