High throughput transcriptomic profiling of the embryonic C. elegans intestine with single-cell resolution
Ontology highlight
ABSTRACT:
ORGANISM(S): Caenorhabditis elegans
PROVIDER: GSE344299 | GEO | 2026/08/24
REPOSITORIES: GEO
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Project description:Intestinal development during late embryogenesis and early posthatch has a long term influence on digestive and absorptive capacity in chickens. The objective of this research was to obtain a global view of intestinal gene expression from late embryogenesis until 2 weeks posthatch with a focus on genes involved in nutrient uptake. Experiment Overall Design: Small intestine from male chicks from two different lines(A and B) were collected on embryonic days 18 (e18) and 20 (e20), day of hatch, and days 1, 3, 7, and 14 posthatch. There are two sample pools designed for lines and different times.
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Project description:modENCODE_submission_457 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: JR1130 (engineered, target gene elt-2 tagged by GFP); Tissue: intestinal cells; Developmental Stage: Mixed stage of embryos 20dC; Genotype: wIs84 [elt-2::GFP]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain JR1130 (engineered, target gene elt-2 tagged by GFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue intestinal cells
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