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CD39-stratified CD8+ tumor-infiltrating lymphocytes from head and neck squamous cell carcinoma profiled by bulk RNA sequencing after ex vivo anti-PD-1 and polydatin treatment

ABSTRACT: Tumor-infiltrating lymphocytes were recovered from six untreated head and neck squamous cell carcinoma surgical specimens and pooled. CD8+ TILs were sorted by flow cytometry into CD39-negative, CD39-medium and CD39-high populations and cultured for 72 h under four conditions: vehicle only, anti-PD-1 (nivolumab), polydatin (a G6PD inhibitor), and the combination of anti-PD-1 and polydatin. Bulk RNA sequencing of the twelve resulting libraries was used to determine how CD39 expression level stratifies the transcriptional response to combined checkpoint blockade and metabolic inhibition.

ORGANISM(S): Homo sapiens

PROVIDER: GSE345409 | GEO | 2026/09/21

REPOSITORIES: GEO

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The paradoxical significance of CD39+CD8+ T cells in clear cell renal cell carcinoma [scRNA-seq]

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