Investigating PRELP–TLR2 interactions in the transcriptional control of melanoma immunogenicity

ABSTRACT: Proline/arginine-rich end leucine-rich repeat protein (PRELP) is an extracellular matrix-associated protein involved in tissue remodeling and immune regulation. This study investigates PRELP-associated transcriptional changes in human melanoma using bulk RNA sequencing of PRELP-low, PRELP-high, and PRELP + Toll-like receptor 2 (TLR2) co-transfected BUF1088 cells. PRELP-high cells were generated by experimental PRELP overexpression, while the PRELP + TLR2 group was generated by co-transfection with PRELP and TLR2 expression constructs. Inclusion of the co-transfected samples enables investigation of how TLR2 expression influences PRELP-associated transcriptional programs. Comparative analysis of PRELP-high versus PRELP-low cells revealed broad transcriptomic remodeling, including increased expression of interferon-responsive genes and enrichment of JAK–STAT signaling, chemokine signaling, and MHC class I antigen processing and presentation pathways. These data support a role for PRELP in promoting tumor-intrinsic immune-reactive transcriptional programs and provide a resource for investigating the relationship between extracellular matrix components, TLR2 signaling, and melanoma immune regulation. This work was supported by German Research Foundation (DFG) (KS): 558635546 and EFRE Immu-pATienT (BS, CW): ZS/2024/01/183830.

ORGANISM(S): Homo sapiens

PROVIDER: GSE347202 | GEO | 2026/09/28

REPOSITORIES: GEO