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Effect of disA overexpression on BCG transcriptome

ABSTRACT: This study investigates the transcriptomic consequences of graded intracellular cyclic di-AMP (c-di-AMP) production in Mycobacterium bovis BCG Danish 1331. Wild-type BCG Danish 1331 (BCG-WT) was used as the parental reference strain. Two recombinant strains overexpressing the Mycobacterium tuberculosis diadenylate cyclase gene disA (Rv3586) were generated. In rBCG-V1, disA is expressed from the episomal plasmid pSD5-hsp60 under the control of the constitutive hsp60 promoter. rBCG-V2 carries an extrachromosomal recombinant plasmid, pKSB-hsp60-disA, containing two tandem copies of the hsp60-disA expression cassette, together with duplicated kanR and oriM sequences, while the chromosomal cdnP locus remains intact. These engineered strains generate progressively elevated intracellular c-di-AMP levels, with rBCG-V2 exhibiting higher c-di-AMP production than rBCG-V1. RNA sequencing was performed to characterize the global transcriptional changes associated with increasing c-di-AMP levels by comparing rBCG-V1 and rBCG-V2 with the parental BCG-WT strain.

ORGANISM(S): Mycobacterium tuberculosis variant bovis BCG

PROVIDER: GSE347518 | GEO | 2026/09/21

REPOSITORIES: GEO

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