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RNA-seq analysis of TGF-beta1-stimulated human RPTEC/TERT1 cells treated with mesenchymal stem cell-conditioned media

ABSTRACT: Renal proximal tubular epithelial cell responses to mesenchymal stem cell-conditioned medium were examined in a TGF-beta1-induced profibrotic model. Human RPTEC/TERT1 cells were assigned to four conditions: untreated cells without TGF-beta1 or conditioned medium (NC); cells exposed to 10 ng/mL recombinant human TGF-beta1 for 24 h, followed by complete medium replacement with conditioned medium from immortalized human adipose-derived mesenchymal stem cells transfected with a negative-control miRNA mimic (TN) or a miR-29a-3p mimic (T29). TGF-beta1 was maintained at 10 ng/mL throughout the subsequent 48 h conditioned-medium treatment. Bulk mRNA sequencing was used to characterize condition-associated transcriptomic changes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE348189 | GEO | 2026/09/23

REPOSITORIES: GEO

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