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Transcriptome profiling of the spinal dorsal horn in a rat bone cancer pain model (Sham vs. BCP, part of a study on dexmedetomidine and the Nrf2/Slc7a11/ferroptosis pathway)

ABSTRACT: Bone cancer pain (BCP) is a major source of cancer-related suffering and is difficult to manage with opioid-based therapy. This dataset contains bulk RNA-seq data of the spinal dorsal horn from a rat BCP model, generated as part of a study investigating whether dexmedetomidine alleviates BCP by inhibiting spinal ferroptosis via the Nrf2/SLC7A11 pathway. BCP was induced by injecting Walker 256 mammary gland carcinoma cells into the left tibial bone marrow cavity of female Sprague-Dawley rats; sham-operated rats receiving inactivated Walker 256 cells served as controls (n = 3 per group). Spinal dorsal horn tissues were collected 15 days after inoculation. Compared with the sham group, 1072 genes were differentially expressed in the BCP group (848 up-regulated and 224 down-regulated; |log2 fold change| > 0.5, P < 0.05), with Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showing significant enrichment of the ferroptosis pathway. These data identify ferroptosis as a core pathogenic pathway in the spinal dorsal horn of BCP rats. Funding: Suzhou Applied Basic Research (medical and health) Science and Technology Innovation Project (SYWD2024090).

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE348257 | GEO | 2026/09/25

REPOSITORIES: GEO

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Project description:The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and low responders (LR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in spinal dorsal horn induced by 2Hz/100Hz electroacupuncture in HR and LR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in dorsal horn. Transcriptome profiling analysis found that some co-regulated genes related with electroacupuncture or 2Hz or 100Hz freqencies. These co-regulated genes were plasticity-related by GO analysis. We also found some special regulated genes in HR vs. LR in 2Hz/100Hz electroacupuncture stimulation. These results suggested that neurotransmitter system and cytokine different between HR with LR in 2Hz electroacupuncture. But in 100Hz electroacupunture, there were many different regulated genes related with ribosome between HR with LR, which needs more studies to research the function and may play an important role in HR vs. LR by 100Hz electroacupuncture. Keywords: Transcriptome analysis Rats were exposed to different frequencies (2Hz or 100Hz) electroacupuncture stimlation for 30 min and nociceptive testing and returned to home cages for 1 hours before sacrificed. According to the sensitivity of the analgesic response to 2Hz or 100Hz EA stimulation, the rats divided into four groups: 2Hz-HR group, 2Hz-LR group, 100Hz-HR group and 100Hz-LR group. Subsequently analyzed their dorsal horn transcript profile using cDNA microarrays, the rats were without receiving electroacupuncture as control. The tissue of dorsal horn (DH) of the fifth and sixth lumbar (L5 and L6) of four or five rats were selected for transcript analysis in each group. The five control rats were mixed and labeled with cy5, each rat of experiment groups was labeled with cy3.

Dataset Information

Transcriptome profiling of the spinal dorsal horn in a rat bone cancer pain model (Sham vs. BCP, part of a study on dexmedetomidine and the Nrf2/Slc7a11/ferroptosis pathway)

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