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RNA-seq of NB4 cells treated with the PKM2 inhibitor PKM2-IN-1

ABSTRACT: To investigate the transcriptional changes induced by PKM2 inhibition in acute myeloid leukemia (AML), we performed RNA sequencing (RNA-seq) in NB4 cells treated with the PKM2-specific allosteric inhibitor PKM2-IN-1. NB4 cells were treated with 0.25 μM PKM2-IN-1 or an equal volume of DMSO (control) for 48 h. Total RNA was isolated from three biological replicates per condition. Stranded RNA sequencing libraries were constructed and sequenced on the DNBSEQ-T7 platform in paired-end 150 bp mode. Raw reads were processed using Trimmomatic, and gene-level counts were obtained with featureCounts. Differentially expressed genes were identified using edgeR, and GSEA was performed to explore pathway enrichment. This dataset enables the identification of PKM2-regulated genes and provides insights into the role of PKM2 in AML cell metabolism and malignancy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE349035 | GEO | 2026/09/29

REPOSITORIES: GEO

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