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Effect of Doxycycline on Wolbachia and Brugia malayi adult female worms in vivo

ABSTRACT: Doxycycline treatment affects gene expression in Wolbachia and Brugia malayi adult female worms in vivo

ORGANISM(S): Wolbachia endosymbiont strain TRS of Brugia malayi Brugia malayi Wuchereria bancrofti Onchocerca volvulus

PROVIDER: GSE34976 | GEO | 2012/01/16

SECONDARY ACCESSION(S): PRJNA151107

REPOSITORIES: GEO

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Effect of Doxycycline on Wolbachia and Brugia malayi adult female worms in vivo

Project description:Doxycycline treatment affects gene expression in Wolbachia and Brugia malayi adult female worms in vivo Two biological replicates of female RNA used for hybridization, in duplicate, to examine the gene expression changes in Wolbachia and Brugia

2012-01-16 | E-GEOD-34976 | biostudies-arrayexpress

Identification of cross-species microRNA-based Communication in Brugia malayi with its endosymbiont Wolbachia

Project description:Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. The largest identified cluster is downregulated in adult females and enriched in zinc finger domains, helicase domains, and DNA binding domains suggesting this microRNA cluster may have regulatory control over a large proportion of adult female specific mRNA genes. MicroRNA-like molecules are identified as produced by the Wolbachia endosymbiont, providing evidence for direct nucleic acid-based interdomain communication between filarial nematodes and their bacterial obligate endosymbiont.

2022-01-15 | GSE143730 | GEO

L3-L4 Developmental Transcriptome of the human parasitic nematode Brugia malayi and its Wolbachia endosymbiont

Project description:Lymphatic filarial nematodes maintain a mutualistic association with the intracellular bacterium Wolbachia. Wolbachia populations expand following infection of the mammalian host, to support larval growth and development. Utilizing transcriptomic data from Brugia malayi over the first two weeks post-infection, we present an analysis of the biochemical pathways that are involved in Wolbachia population growth and regulation in support of larval development. In Wolbachia, we observe coordinated regulation of carbon metabolism with an alternating pattern of glycolysis and TCA cycle pathways reminiscent of the ‘Warburg effect’. Wolbachia's purine, pyrimidine and haem biosynthesis and Type IV secretion pathways are also upregulated and correlate with the upregulation of the nematode’s DNA replication pathway. In the nematode we observe up-regulation of the autophagy pathway, a key regulator of Wolbachia populations. These findings support a key role for nucleotide and haem provisioning from Wolbachia in support of the larval growth and development of its nematode host.

2021-06-30 | GSE132848 | GEO

Wolbachia endosymbiont of Brugia malayi

Project description:Wolbachia endosymbiont of Brugia malayi Genome sequencing

| PRJNA286859 | ENA

Brugia malayi

Project description:Effect of Doxycycline on Wolbachia and Brugia malayi adult female worms in vivo

| PRJNA151107 | ENA

Wolbachia endosymbiont of Brugia malayi strain:TRS

Project description:Transcriptomic Investigation of Wolbachia of Brugia malayi

| PRJNA376277 | ENA

Effects of doxycycline on gene expression in Wolbachia and Brugia malayi adult female worms in vivo.

Project description: Not available

| S-EPMC3352068 | biostudies-literature

Microarray analysis of Brugia malayi microfilariae-derived extracellular vesicles (Evs) and supernatants

Project description:We report our microarray analysis of Brugia malayi microfilariae-derived miRNA comparing parasite-derived EVs and supernatants Microarray analysis was performed using isolated RNA from three biological replicates of Brugia malayi microfilariae with a focus on the parasite-derived EVs and supernatant

2021-01-12 | GSE157226 | GEO

Profiling of gender based transcripts of Brugia malayi

Project description:Transcriptional profiling of adult males and females of the lymphatic dwelling filarial parasite Brugia malayi Keywords: Gender based transcripts, filaria

2010-12-30 | GSE12944 | GEO

N-glycosite mapping in Brugia malayi female worms, male worms, and microfilariae

Project description:N-linked glycosylation is a critical post translational modification of eukaryotic proteins. N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions. Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite. In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS. We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae. Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites. Our data identified 582 N-linked glycoproteins with 1273 N-glycosites. Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins. Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level. These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.

2023-04-23 | PXD039002 | Pride

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