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Gene expression from MDA-MB-231

ABSTRACT: Gene expression from MDA-MB-231 cells shControl and shLOXL2.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35600 | GEO | 2012/02/09

SECONDARY ACCESSION(S): PRJNA152521

REPOSITORIES: GEO

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Gene expression from MDA-MB-231

Project description:Gene expression from MDA-MB-231 cells shControl and shLOXL2. We analyzed 3 arrays from each condition: shControl and shLOXL2.

2012-02-09 | E-GEOD-35600 | biostudies-arrayexpress

Expression profiles of MDA-MB-231, MDA-231 S1a and S1b

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Expression data from human breast cancer cells MDA-MB-231-Luc knockdown for RRAS2 expression.

Project description:We used microarrays to investigate gene expression changes induced by the inhibition of RRAS2 expression using shRNA techniques to stably knockdown the endogenous transcripts of this GTPase in human MDA-MB-231-Luc cells. MDA-MB-231-Luc shControl (MDA-Control) and RRAS2-deficient (KDRRAS2.p1) in exponential growth phase were selected for RNA extraction and and hybridization on Affymetrix microarrays.

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Project description:To determine the effect ALDH1A3 expression on global gene expression in MDA-MB-231 cells and MDA-MB-468 cells

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ALDH1A3-mediated gene expression in MDA-MB-231 cells and MDA-MB-468 cells

Project description:To determine the effect ALDH1A3 expression on global gene expression in MDA-MB-231 cells and MDA-MB-468 cells In MDA-MB-231 cells, ALDH1A3 was overexperssed (have low endogenous levels of ALDH1A3) and compared to MSCV empty vector control. In MDA-MB-468 cells that have high endogenous levels of ALDH1A3, ALDH1A3 expresion was reduced with ALDH1A3 shRNA1 and compared to scramble shRNA control.

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Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells

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Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells

Project description:To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).

2012-02-06 | E-GEOD-35511 | biostudies-arrayexpress

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