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Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness

ABSTRACT: Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. Tthe phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24.

ORGANISM(S): Oryza sativa Oryza sativa Japonica Group

PROVIDER: GSE36271 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA153103

REPOSITORIES: GEO

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Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness

Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.

2013-12-31 | E-GEOD-36271 | biostudies-arrayexpress

RNA-seq of Arabidopsis seedlings with the treatment of GR24-5DS

Project description:This dataset provides deep-profiling of the Arabidopsis transcriptome of mRNA of 7-day whole seedling on treatment with strigolactone (SL) isomer GR24-5DS. To understand the transcriptome response and genes regulated by SL, we used Columbia (Col0) ecotype. 7-day old seedlings are treated with 2.5uM GR24-5DS for 3hrs and samples are frozen immediately after treatment. 6 samples: 3 biological replicates Control untreated; 3 biological replicates treated with GR24-5DS.

2022-09-20 | E-MTAB-11329 | biostudies-arrayexpress

Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor

Project description:The F-box protein MORE AXILLARY GROWTH 2 (MAX2) is a central component in the signaling cascade of strigolactones (SLs) as well as of the smoke derived karrikins (KARs) and the so far unknown endogenous KAI2 ligand (KL). The two groups of molecules are involved in overlapping and unique developmental processes, and signal-specific outcomes are attributed to perception by the paralogous α/β-hydrolases DWARF14 (D14) for SL and KARRIKIN INSENSITIVE 2/ HYPOSENSITIVE TO LIGHT (KAI2/HTL) for KAR/KL. Additionally, depending on which receptor is activated, specific members of the SUPPRESSOR OF MAX2 1 (SMAX1) – LIKE (SMXL) 6, 7, 8 clade control KAR/KL and SL responses respectively. As proteins that function in the same signal transduction pathway often occur in large protein complexes, we aimed at discovering new players of the MAX2, D14 and KAI2 protein network by tandem affinity purification using Arabidopsis cell cultures. When using MAX2 as a bait, various proteins were co-purified among which general components of the Skp1-Cullin-F-box complex and members of the CONSTITUTIVE PHOTOMORPHOGENIC 9 signalosome. Here, we report the identification of a novel interactor of MAX2, a type 5 serine/threonine protein phosphatase, designated PHYTOCHROME-ASSOCIATED PROTEIN PHOSPHATASE 5 (PAPP5). Quantitative affinity purification pointed at PAPP5 as being more present in KAI2 rather than D14 protein complexes. In agreement, mutant analysis suggests that PAPP5 modulates KAR/KL-dependent seed germination in suboptimal conditions and seedling development. Additionally, PAPP5 was found to dephosphorylate MAX2 in vivo independent of the synthetic strigolactone analog, rac-GR24. Together, by analyzing the protein complexes to which MAX2, D14 and KAI2 belong, we revealed a new MAX2 interactor that might act through dephosphorylating MAX2 to control mainly KAR/KL signaling.

2021-01-06 | PXD015657 | Pride

E-GEOD-36271

Project description:Transcription profiling by array of rice dwarf d10-1 and d14-1 mutants treated with GR24, a synthetic strigolactone analog

2015-11-23 | E-GEOD-36271 | ExpressionAtlas

Gibberellin-induced gene expression in maize mesocotyl under deep-sowing condition

2009-03-07 | GSE15048 | GEO

Gibberellin-induced gene expression in maize mesocotyl under deep-sowing condition

Project description:In order to elucidate the molecular mechanism of gibberellin (GA)-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combing Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying GA promotion of maize mesocotyl elongation. These studies showed that the GA receptor GID1, the transcriptional factor MYB, and the genes encoding DELLA protein DWRF8, kinases, Raf, LRR, RLCK, and involved in flavonoid biosynthesis, aminosugars metabolism, cell wall synthesis and modification, might play critical roles in maize mesocotyl elongation. In two independent experiments, we generate 10-day-old maize mesocotyl-specific gene expression profiles through comparing genome-wide expression patterns of GA3 treatment and UCZ (a GA biosynthesis inhibitor) treatment under 20 cm deep-sowing condition in darkness by using 17,555 Affymetrix maize whole genome array.

2009-03-07 | E-GEOD-15048 | biostudies-arrayexpress

Plant-derived compounds strigolactone GR24 and pinosylvin modulate gene expression in rat L6 skeletal muscle cells

Project description:We investigated the effects of novel SIRT1 activating plant-derived compounds strigolactone GR24 and pinosylvin on gene expression in rat L6 skeletal muscle myotubes. Resveratrol was included in analyses as a reference compound.

2017-12-18 | GSE90833 | GEO

A dynamic transcriptome underling the light inhibition of mesocotyl elongation in rice seedling

Project description:The mesocotyl, a structure separating the grass seedling cotyledon from the coleoptile, exerts a major influence over the seedling’s ability to emerge. Its elongation is inhibited by light. A transcriptomic comparison between mesocotyl tissue which had developed in the dark and which had been exposed to light identified a large number of differentially transcribed genes (DTGs)

2021-08-21 | GSE136318 | GEO

Gene expression profiles following GR24, smoke water and KAR1 treatments in Nicotiana benthamiana seedlings

Project description:GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common α,β unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses.

2010-02-25 | GSE20520 | GEO

Gene expression profiles following GR24, smoke water and KAR1 treatments in Nicotiana benthamiana seedlings

Project description:GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common Î±,Î² unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses. Control (untreated), GR24 (10-7M), smoke water (1:1000 dilution) and butenolide (10-7M) gene expression profiles were evaluated on three week old seedlings, in two independent experimental trials.

2010-04-22 | E-GEOD-20520 | biostudies-arrayexpress

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