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The AIN-centered DCN of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups

ABSTRACT: Anterior interpositus nucleus (AIN) is a proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remains unknown. To elucidate molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delayed-type eyeblink conditioning Keywords: 3-d paired training, sham negative control, delayed-type eyeblink conditioning of mice, anterior interpositus nucleus-centered deep cerebellar nuclei

ORGANISM(S): Mus musculus

PROVIDER: GSE3651 | GEO | 2006/04/04

SECONDARY ACCESSION(S): PRJNA94283

REPOSITORIES: GEO

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The AIN-centered DCN of delayed-type eyeblink conditioned mice

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Transcription profiling of mouse AIN-centered DCN of delayed-type eyeblink conditioned mice (GEO Super Series)

Project description:This SuperSeries comprises the following subset Series:; GSE3651: The AIN-centered DCN of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups; GSE3652: The AIN-centered DCN of delayed-type eyeblink conditioned mice: 7-d paired and 7-d unpaired training groups; Anterior interpositus nucleus (AIN) is a proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remains unknown. To elucidate molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delayed-type eyeblink conditioning; The data are not directly comparable between GSE3651 and GSE3652, given the different experimental time periods and amounts of cRNA used for hybridizations. Experiment Overall Design: Refer to individual Series

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Transcription profiling of mouse Anterior interpositus nucleus centred DCN from mice with of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups

Project description:Anterior interpositus nucleus (AIN) is a proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remains unknown. To elucidate molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delayed-type eyeblink conditioning Experiment Overall Design: 3-d paired training group: Mice received a surgery for implanting four Teflon-coated stainless-steel wires in their left eyelid and a headstage on their head. After 3daysâ?? recovery, they were trained with paired paradigm of conditioned stimulus (CS) and unconditioned stimulus (US) for 3days: A 352-ms tone CS (1kHz, 83~85dB) was delivered first and a 100ms periorbital shock US (100kHz square pluses) were delivered with 252ms after the onset of CS, and they co-terminated. After the last trial was given, anterior interpositus was immediately sampled in 10 to 30 min from the sacrifice. Experiment Overall Design: Sham negative control group: Mice received a surgery for implanting four Teflon-coated stainless-steel wires in their left eyelid and a headstage on their head. After recovery, anterior interpositus nucleus-centered deep cerebellar nuclei were immediately sampled in 10 to 30 min from the sacrifice. Experiment Overall Design: ~10-15 anterior interpositus-centered deep cerebellar nuclei ipsilateral to the eye implanted with four wires were pooled into the same sham control or training group and subjected to microarray analysis.

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Transcription profiling of mouse anterior interpostus nucleus (AIN) in delayed type eyeblink conditioned mice after training to investigate memory formation of eyeblink conditioning

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Project description:The AIN-centered DCN of delayed-type eyeblink conditioned mice

| PRJNA93869 | ENA

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