Comparison of muscle stem cell preplates and myoblasts.
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE3769 | GEO | 2005/12/09
SECONDARY ACCESSION(S): PRJNA93951
REPOSITORIES: GEO
Similar Datasets
Project description:Comparison of muscle stem cell preplates and myoblasts. Experiment Overall Design: this experiment include 2 samples and 2 replicates
2008-06-12 | E-GEOD-3769 | biostudies-arrayexpress
Project description:Quiescent muscle stem cells, commonly known as satellite cells, are crucial for muscle repair and can convert into committed myoblasts capable of proliferation and differentiation upon in vitro culture. However, following prolonged propagation, myoblasts frequently lose myogenic differentiation capacity, limiting their utility in research and clinical applications. Here, we demonstrate that exposing committed mouse myoblasts to a small-molecule cocktail elicits their conversion into expandable and heterogeneous myogenic progenitor cells (MPCs), comprised of muscle stem, progenitor and differentiated cells. Utilizing a new dual-fluorescent reporter for Pax7 and MyoD, we demonstrate that the small molecules de-differentiate Pax7+/MyoD+ myoblasts into Pax7+/MyoD- satellite-like cells within days. This conversion is characterized by upregulation of signaling pathways associated with satellite cells including Notch, Calcitonin and EGFR. Accordingly, genetic ablation of Notch1-expressing cells abrogated MPC cultures but not committed myoblasts. Furthermore, a comparison with in vivo-derived freshly isolated and activated satellite cells through single-cell transcriptomics revealed that the stem cell subset in MPCs shares common features with both cell types, particularly with a sub-population of activated satellite cells. Collectively, our study presents a method to de-differentiate myoblasts into MPCs harboring satellite cell attributes in vitro, offering a new avenue for studying myogenesis and advancing muscle disease therapeutics.
2026-04-30 | GSE295091 | GEO
Project description:Quiescent muscle stem cells, commonly known as satellite cells, are crucial for muscle repair and can convert into committed myoblasts capable of proliferation and differentiation upon in vitro culture. However, following prolonged propagation, myoblasts frequently lose myogenic differentiation capacity, limiting their utility in research and clinical applications. Here, we demonstrate that exposing committed mouse myoblasts to a small-molecule cocktail elicits their conversion into expandable and heterogeneous myogenic progenitor cells (MPCs), comprised of muscle stem, progenitor and differentiated cells. Utilizing a new dual-fluorescent reporter for Pax7 and MyoD, we demonstrate that the small molecules de-differentiate Pax7+/MyoD+ myoblasts into Pax7+/MyoD- satellite-like cells within days. This conversion is characterized by upregulation of signaling pathways associated with satellite cells including Notch, Calcitonin and EGFR. Accordingly, genetic ablation of Notch1-expressing cells abrogated MPC cultures but not committed myoblasts. Furthermore, a comparison with in vivo-derived freshly isolated and activated satellite cells through single-cell transcriptomics revealed that the stem cell subset in MPCs shares common features with both cell types, particularly with a sub-population of activated satellite cells. Collectively, our study presents a method to de-differentiate myoblasts into MPCs harboring satellite cell attributes in vitro, offering a new avenue for studying myogenesis and advancing muscle disease therapeutics.
2026-04-30 | GSE295302 | GEO
Project description:Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of both transcription factors are affected in satellite cell-derived myoblasts (MyoD-/-, p53 -/-). Experiment Overall Design: this experiment include 3 samples and 25 replicates
2008-06-12 | E-GEOD-3244 | biostudies-arrayexpress
Project description:Skeletal muscle cells (myoblasts - human fetal cells from 17 week old embryo) Keywords: other
2005-09-01 | GSE3230 | GEO
Project description:Satellite cells play an important role in post-natal growth and regeneration of skeletal muscle. They can be defined as a population adult muscle stem cells based on their self renewal capability and ability to differentiate into skeletal muscle fibers. Functional Retinoblastoma protein (pRb) is essential for the process of skeletal muscle differentiation in satellite cell derived primary myoblasts. Furthermore, the biochemical function of pRb is largely associated with its ability to interact with chromatin modifying factors such as histone deacetylases (HDACs) and histone methyltransferases thus inhibiting transcription of target gene promoters. Hence, expression profiling of pRb null primary myoblasts and myotubes will provide a global picture of the downstream targets of pRb transcriptional regulation in relation to cell cycle control, apoptosis inhibition, and muscle differentiation. Keywords: other
2008-02-13 | GSE10428 | GEO
Project description:Comparison of primary myoblasts isolated from 8- and 23-month-old DBA/2JNIA mice. Keywords: other
2005-01-10 | GSE273 | GEO
Project description:Conversion of Committed Mouse Myoblasts into Muscle Stem Cells Using Small Molecules
| PRJNA1254143 | ENA
Project description:Direct lineage reprogramming provides a unique system to study cell fate transitions and unearth molecular mechanisms that safeguard cellular identity. We previously reported on direct conversion of mouse fibroblasts into induced myogenic progenitor cells (iMPCs) by transient MyoD overexpression in concert with small molecules treatment. Here we employed integrative multi-omic approaches to delineate the molecular landscape of fibroblast reprogramming into iMPCs in comparison to transdifferentiation into myogenic cells solely by MyoD overexpression. Utilizing bulk RNA-sequencing and mass spectrometry, we uncovered molecular regulators and pathways that endow a myogenic stem cell identity on fibroblasts only in the presence of small molecule treatment. In addition, we demonstrate that Pax7+ cells in iMPCs share molecular attributes with myoblasts, however in addition express unique genes, proteins and pathways that are indicative of a more activated satellite cell-like state in vitro. Collectively, this study charts a molecular blueprint for reprogramming fibroblasts into muscle stem and progenitor cells and further establishes the fidelity of stable iMPC cultures in capturing skeletal muscle regeneration in vitro for disease modeling and basic research applications.
2022-05-19 | PXD029379 | Pride
Project description:Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells. Keywords: other
2005-12-09 | GSE3773 | GEO