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Expression data from Drosophila embryos, control vs. dMyc+

ABSTRACT: dMyc is a conserved transcription factor that controls growth and proliferation by regulating its target genes. We used Affymetrix microarray to identify and classify targets of dMyc during Drosophila embryogenesis.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE38529 | GEO | 2012/09/15

SECONDARY ACCESSION(S): PRJNA168107

REPOSITORIES: GEO

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Expression data from Drosophila embryos, control vs. dMyc+

Project description:dMyc is a conserved transcription factor that controls growth and proliferation by regulating its target genes. We used Affymetrix microarray to identify and classify targets of dMyc during Drosophila embryogenesis. RNA was extracted from 0-24 hour old Drosophila embryos in control (Gal 4 expressing animals) and Myc+ (flies over-expressing dMyc under UAS enhancer) to identify genomic targets od dMyc during embryogenesis

2012-09-15 | E-GEOD-38529 | biostudies-arrayexpress

RNA-seq on Drosophila wing imaginal discs after Myc RNAi or overexpression

Project description:RNA-seq was performed to confirm the effect of dMyc overexpression or RNAi repression on conserved Myc targets in the Drosophila wing disc.

2022-04-26 | E-MTAB-9328 | biostudies-arrayexpress

dSirt6 Regulates Lifespan in Drosophila

Project description:Sirt6 is a multifunctional enzyme that regulates diverse cellular processes such as metabolism, DNA repair, and aging. Overexpressing Sirt6 extends lifespan in mice, but the underlying cellular mechanisms are unclear. Drosophila melanogaster are an excellent model to study genetic regulation of lifespan; however, despite extensive study in mammals, very little is known about Sirt6 function in flies. Here, we characterized the Drosophila ortholog of Sirt6, dSirt6, and examined its role in regulating longevity. dSirt6 is a nuclear and chromatin-associated protein with NAD+-dependent histone deacetylase activity. dSirt6 overexpression in flies produces robust lifespan extension in both sexes, while reducing dSirt6 levels shortens lifespan. dSirt6 OE flies have normal food consumption and fertility, but increased resistance to oxidative stress and reduced protein synthesis rates. Transcriptomic analyses reveal that dSirt6 OE reduces expression of genes involved in ribosome biogenesis, including many dMyc target genes. dSirt6 OE partially rescues many effects of dMyc OE, including increased nuclear size, up-regulation of ribosome biogenesis genes, and lifespan shortening. Lastly, dMyc haploinsufficiency does not convey additional lifespan extension to dSirt6 OE flies, suggesting dSirt6 OE is upstream of dMyc in regulating lifespan. Our results provide new insight into the mechanisms by which Sirt6 overexpression leads to longer lifespan.

2021-12-23 | GSE191320 | GEO

Drosophila early embryos LC-MSMS

Project description:Cytoplasmic polyadenylation is a mechanism to promote mRNA translation in a wide variety of biological contexts.The conserved RNA-binding protein family CPEB has been shown to mediate canonical cytoplasmic polyadenylation of target transcripts. We have previously reported evidence for RNA-interference factor Dicer-2 as a component of a non-canonical complex, that operates independent of CPEB in Drosophila. In this study, we investigate Dicer-2 mRNA targets and protein co-factors in cytoplasmic polyadenylation. Using RIP‐Seq analysis we identify hundreds of potential Dicer-2 target transcripts, ~60% of which were previously found as targets of the cytoplasmic poly(A) polymerase Wispy, suggesting widespread roles of Dicer-2 in cytoplasmic polyadenylation. Large-scale immunoprecipitation and mass spectrometry revealed Ataxin-2 and Twenty-four among the high-confidence interactors of Dicer-2. Complex analyses indicated that both factors form an RNA‐independent complex with Dicer‐2, and mediate interactions of Dicer‐2 with Wispy. Functional poly(A)‐test analyses showed that Twenty‐four and Ataxin-2 are required for cytoplasmic polyadenylation of a subset of Dicer‐2 targets. Our results reveal components of a novel cytoplasmic polyadenylation complex that operates during Drosophila early embryogenesis.

2022-08-30 | PXD030281 | Pride

Absolute quantification of proteins and histone modifications in Drosophila embryos

Project description:Mass spectrometry analysis for absolute and relative quantification of the nuclear proteome and of methyl-marks on selected lysine residues in histone H3 during two stages of Drosophila embryogenesis: 2-4 hr and 14-16 hr old.

2023-02-01 | PXD015306 | Pride

Modular evolution of the Drosophila metabolome

Project description:In this study, we look at both targeted and untargeted metabolomic data from both sexes and 11 species of Drosophila. For the targeted analysis, we also looked at two ages to understand conserved changes with age in the Drosophila genus.

2021-09-01 | ST001926 | MetabolomicsWorkbench

Gene expression profiling of dMyc null Drosophila larvae

Project description:Myc oncoproteins are essential regulators of the growth and proliferation of mammalian cells. In Drosophila the single ortholog of Myc (dMyc), encoded by the dm gene, influences organismal size and the growth of both mitotic and endoreplicating cells. A null mutation in dm (dm4) results in attenuated endoreplication and growth arrest early in larval development. Gene expression analysis indicates that loss of dMyc leads to decreased expression of genes required for ribosome biogenesis and protein synthesis. Keywords: cDNA expression microarray, mutant analysis

2008-01-22 | GSE9729 | GEO

Drosophila melanogaster

Project description:Expression data from Drosophila embryos, control vs. dMyc+

| PRJNA168107 | ENA

Drosophila melanogaster

Project description:Gene expression profiling of dMyc null Drosophila larvae

| PRJNA103647 | ENA

Laser wounded vs unwounded syncytium Drosophila embryos

Project description:Transcriptional profiling of laser wounded syncytial Drosophila embryos compared with control unwounded embryos. Early Drosophila embryos (Nuclear cycle 4-6) were collected, either laser wounded or not wounded, and then collected and processed immediately. Wounded and unwounded embryos express sGMCA (actin reporter)

2020-04-06 | GSE39481 | GEO

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