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Expression data from U2OS ER-E2F1 cells after FOXO knockdown and E2F1 activation

ABSTRACT: Effect of FOXO knockdown on E2F1-mediated transcription

ORGANISM(S): Homo sapiens

PROVIDER: GSE39136 | GEO | 2012/07/06

SECONDARY ACCESSION(S): PRJNA170072

REPOSITORIES: GEO

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Expression data from U2OS ER-E2F1 cells after FOXO knockdown and E2F1 activation

Project description:Effect of FOXO knockdown on E2F1-mediated transcription U2OS cells stably expressing ER-E2F1 were infected with two different lentiviruses both targeting FOXO1 and FOXO3 or control virus encoding scrambled sequence. Twenty four hours post infection medium was replaced to serum-free DMEM for 24 hours. Then medium was replaced to serum-free DMEM with or without 20 nM 4-hydroxy tamoxifen for 6 hours.

2012-07-05 | E-GEOD-39136 | biostudies-arrayexpress

Expression data of U2OS ER-HA-E2F1 cells induced by OHT with and without knockdown of NFYB

Project description:E2F1 induces numerous genes, including transcription factors, upon activation. The transcription factors then further cooperates with E2F1 to regulate the target genes and enhance the transcriptional effect. We used microarrays to analyze genes upregulated upon E2F1 activation by OHT with and without knockdown of NFYB and identified genes that are targets of both transcription factors.

2016-09-09 | GSE61272 | GEO

E2F1 transcription factor knockdown effect on gene expression in LNCaP-abl cells

Project description:Our computational approach identified E2F1 as a potential collaborator of EZH2 in androgen-independent prostate cancer. This experiment is to designed to validate the crosstalking of E2F1 and EZH2 pathways. We showed that majority of the EZH2-induced genes in androgen-independent prostate tumor cells are in downstream of E2F1, providing insight into the EZH2-E2F1 collaborative regulatory pathway.

2016-01-22 | GSE56331 | GEO

E2F1 ChIP-Seq on mouse hepatocytes overexpressing E2F1

Project description:E2F transcription factors are known regulators of the cell cycle, proliferation, apoptosis and differentiation. We reveal an essential role for E2F1 in liver through the regulation of glycolysis and lipogenesis. E2F1 deficiency leads to a decreased in glycolysis and de novo synthesis of fatty acids in hepatocytes. ChIP-Seq was performed to determine direct tagets of E2F1 in hepatocytes. We highlight that E2F1 directly binds the promoters of genes implicated in metabolic process and notably key lipogenic genes to control these pathways.

2015-12-01 | GSE74006 | GEO

Foxo signaling declines with age

Project description:We performed RNA-seq for WT and foxo mutant flies, by combining with Dr. Baihua's foxo chip-seq data, we found 101 foxo-repressed and 300 foxo-activated genes. By analyzing expression change of these genes during age, we found foxo-repressed genes became activated, while foxo-activated genes became repressed, suggesting foxo signaling declines with age.

2018-11-14 | GSE122470 | GEO

miRNA expression analysis of E2F1 knockdown cells compared to control siRNA treated cells

Project description:To have a global picture of the miRNAs dependent on the transcription factor E2F1, we assessed small RNA changes, by RNA-sequencing, of HeLa cells transfected with an siRNA targeting E2F1 or a control siRNA.

2021-04-12 | GSE147361 | GEO

Skeletal muscle gene expression after high-fat diet with or without endothelial-directed deletion of FoxO

Project description:In a murine model of high-fat diet, Mx1-Cre was used to induce knockdown of forkhead box O 1,3 and 4 (FoxO 1,3 and 4) in the endothelium of adult mice. Skeletal muscle gene-expression was assessed after 8 weeks of high-fat diet in wild type versus FoxO knockdown mice.

2015-12-01 | GSE72576 | GEO

Expression data from MxCre;E2F1-/-2-/-3f/f Cd11B myeloid cells

Project description:To understand the underlying cause for the observed apoptosis in E2f1-3 deficient myeloid cells. We compared gene expression profiles of Cd11b+ sorted myeloid cells isolated from bone marrow of control (E2F1-/- ) and experimental (Mxcre;E2F1-/-2-/-3f/f ) mice.

2010-12-03 | GSE25825 | GEO

Homo sapiens

Project description:Expression data from U2OS ER-E2F1 cells after FOXO knockdown and E2F1 activation

| PRJNA170072 | ENA

DK009: FOXO target gene CTDSP2 inhibits cell cycle progression through regulation of p21-Cip1/Waf1

Project description:Activity of Forkhead box O (FOXO) transcription factors is inhibited by PI3K-PKB/Akt signalling to control a variety of cellular processes including cell cycle progression. Through comparative analysis of a number of microarray datasets, we identified a set of genes commonly regulated by FOXO and PI3K/PKB, which includes Carboxyl-Terminal Domain Small Phosphatase 2 (CTDSP2). We validated CTDSP2 as a genuine FOXO target gene and show that ectopic CTDSP2 can induce cell cycle arrest. We analysed transcriptional regulation after CTDSP2 expression and identified extensive regulation of genes involved in cell cycle progression, which depends on the phosphatase activity of CTDSP2. Most notably regulated genes are p21Cip1/Waf1 and E2F1, both implicated in S-phase entry. We show that p21Cip1/Waf1 is regulated by CTDSP2 in a p53-independent manner and that p21Cip1/Waf1 upregulation results in decreased cyclin/CDK2 and cyclin/CDK6 activity. Thus, we identify FOXO-dependent CTDSP2 regulation as a novel regulatory mechanism for inhibiting proliferation in the absence of growth factor/PI3K signalling.

2015-05-29 | GSE58947 | GEO

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