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Salmonella wild type in LB, 2mM indole until OD1 and 2mM indole for 15 minutes

ABSTRACT: Transcriptional profiling of Salmonella Typhimurium SL1344 wild type grown to OD600=1 in LB alone or in LB supplemented with 2mM Indole, or with 2mM indole for 15 min (called shock). The goal is to determine Salmonella response to indole at different time points.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109

PROVIDER: GSE39760 | GEO | 2013/01/30

SECONDARY ACCESSION(S): PRJNA171591

REPOSITORIES: GEO

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Salmonella wild type in LB, 2mM indole until OD1 and 2mM indole for 15 minutes

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type grown to OD600=1 in LB alone or in LB supplemented with 2mM Indole, or with 2mM indole for 15 min (called shock). The goal is to determine Salmonella response to indole at different time points. Different conditions were tested. The reference on each hybridisation is the chromosome DNA of the wild type strain. 4 replicates each for LB and LB + Indole, 6 replicates for indole shock.

2013-01-30 | E-GEOD-39760 | biostudies-arrayexpress

Salmonella wild type and mutant sdiA in LB and 2mM indole

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Salmonella wild type and mutant sdiA in LB and 2mM indole

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type and deletion of the sdiA regulator-encoding gene grown to OD600=1 in LB alone or in LB supplemented with 2mM indole. The goal is to determine Salmonella response to indole and to define the SdiA-regulated genes in these conditions. 4 different conditions tested (wild type in LB, wild type in LB + 2mM indole, sdiA mutant in LB, sdiA mutant in LB + 2mM indole), 4 replicates for each condition. The reference on each hybridisation is the chromosomal DNA of the wild type strain.

2013-01-30 | E-GEOD-36805 | biostudies-arrayexpress

Salmonella wild type and mutant baeR in LB and tungstate

2011-08-30 | GSE28861 | GEO

Salmonella wild type and mutant baeR in LB and tungstate

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type and deleted of the response regulator encoding gene baeR grown to OD600=1 in LB alone or in LB supplemented with 20mM of Sodium Tungstate. The goal is to determine Salmonella response to tungstate and to define the BaeR regulated genes in this conditions. 4 differents conditions tested, the reference on each hybridisation is the chromosomique DNA of the wild type strain. 3 replicates for the wild type on LB, 3 replicates for the baeR on LB, 3 replicates for the wild type on LB+ tungstate and 5 replicates for baeR on LB+tungstate.

2011-08-29 | E-GEOD-28861 | biostudies-arrayexpress

SL1344_hydrogen_peroxide_2mM_DNA

Project description:Gene content in Salmonella enterica sv Typhimurium SL1344 post treatment with 2mM hydrogen peroxide Keywords: parallel sample

2004-05-10 | GSE625 | GEO

Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

Project description:Salmonella wild type in LB, 2mM indole until OD1 and 2mM indole for 15 minutes

| PRJNA171591 | ENA

E. coli K-12 mutants yceP, trpE, and tnaA biofilm cell 24 hr for indole paper

Project description:Gene expression of (i) K-12 yceP biofilms upon addition of 600 µM indole in LB glu medium, (ii) K-12 biofilms due to the trpE mutation in LB glu medium, and (iii) K-12 biofilms due to the tnaA mutation in LB medium. Keywords: indole effect and cell type comparison

2007-05-01 | GSE4562 | GEO

Transcriptome analysis of the Salmonella enterica OmpR regulon

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type and ompR mutant grown to mil-exponential phase in LB. The goal was to define the ompR-regulated genes.

2012-12-01 | GSE35938 | GEO

Minimal vs LB media comparison

Project description:The type of bacterial culture medium is an important consideration during design of any experimental protocol. The aim of this study was to understand the impact of medium choice on bacterial gene expression and physiology by comparing the transcriptome of Salmonella enterica SL1344 after growth in the widely used LB broth or the rationally designed MOPS minimal medium. Transcriptomics showed that after growth in MOPS minimal media, compared to LB, there was increased expression of 42 genes involved in amino acid synthesis and 23 genes coding for ABC transporters. Seven flagellar genes had decreased expression after growth in MOPS minimal medium and this correlated with a decreased motility. In both MOPS minimal medium and MEM expression of genes from SPI-2 was increased and the adhesion of S. Typhimurium to intestinal epithelial cells was higher compared to the levels after growth in LB. However, SL1344 invasion was not significantly altered by growth in either MOPs minimal media or MEM. Expression of SPI-2 was also measured using chromosomal GFP reporter fusions followed by flow cytometry which showed, for the first time, that the reduction in SPI-2 transcript after growth in different media related to a reduction in the proportion of the bacterial population expressing SPI-2. These data highlight the profound differences in the global transcriptome after in vitro growth in different media and show that choice of medium should be considered carefully during experimental design, particularly when virulence related phenotypes are being measured.

2014-07-31 | E-MTAB-1135 | biostudies-arrayexpress

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