Genomics

Dataset Information

0

Virulent and nonvirulent Entamoeba species comparison


ABSTRACT: Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to assess the transcriptional differences between these species/strains with the goal of identifying genes whose expression correlated with a virulence phenotype. We found 415 genes expressed at lower levels in E. dispar and 32 genes with lower expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS. Overall, 29 genes had decreased expression in both the nonvirulent species/strains than the virulent E. histolytica HM-1:IMSS. Interestingly, a number of genes with potential roles in stress response and virulence had decreased expression in either one or both nonvirulent Entamoeba species/strains. These included genes encoding Fe hydrogenase (9.m00419), peroxiredoxin (176.m00112), type A flavoprotein (6.m00467), lysozyme (6.m00454), sphingomyelinase C (29.m00231), and a hypothetical protein with homology to both a Plasmodium sporozoite threonine-asparagine-rich protein (STARP) and a streptococcal hemagglutinin (238.m00054). The function of these genes in Entamoeba and their specific roles in parasite virulence need to be determined. We also found that a number of the non-long-terminal-repeat retrotransposons (EhLINEs and EhSINEs), which have been shown to modulate gene expression and genomic evolution, had lower expression in the nonvirulent species/strains than in E. histolytica HM-1:IMSS. Our results, identifying expression profiles and patterns indicative of a virulence phenotype, may be useful in characterizing the transcriptional framework of virulence. A species experiment design type assays differences between distinct species. Keywords: species_design

ORGANISM(S): Entamoeba histolytica

PROVIDER: GSE4139 | GEO | 2006/02/02

SECONDARY ACCESSION(S): PRJNA95025

REPOSITORIES: GEO

Similar Datasets

| E-SMDB-3589 | biostudies-arrayexpress
| E-SMDB-2766 | biostudies-arrayexpress
| E-GEOD-3207 | biostudies-arrayexpress
| E-MTAB-3525 | biostudies-arrayexpress
2005-08-26 | GSE3207 | GEO
| E-GEOD-43668 | biostudies-arrayexpress
2007-03-01 | GSE6650 | GEO
2008-10-01 | GSE11811 | GEO
| E-MEXP-2504 | biostudies-arrayexpress
| E-GEOD-11811 | biostudies-arrayexpress